ProtParam
User-provided sequence:
10 20 30 40 50 60
MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFR IWMLLWLPLS
70 80 90 100 110 120
VWWKLSNNCI YPLIVSLLVL FLGPIFVLVI CGLSRKRSLS KQLIQFCKEI TENTPSSDPH
130 140 150 160 170 180
DWEVVAANLN SYLYENNVWN TKYFFFNAMV CQEAFRTTLL EPFSLKKDKA AKVKSFKDSV
190 200 210 220 230 240
PYIEEALGVY FTEVEKQWKL FNTEKSWSPV GLEDAKLPKE AYRFKLTWFL KRISNIFMLI
250 260 270 280 290 300
PFLNFLCCIY VSRGMCLLLR TLYLGWILFM LVQGFQNIRV LIMSMEHKMQ FLSTIINEQE
310 320 330 340 350 360
SGANGWDEIA RKMNRYLFEK KAWKNEEFFF DGIDCEWFFN HFFYRVLSAK KSMWPLPLNV
370
ELWPYIKEAQ LSRSEVLLV
References and
documentation are available.
Number of amino acids: 379
Molecular weight: 45165.09
Theoretical pI: 8.78
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2119
Hydrogen H 3212
Nitrogen N 508
Oxygen O 545
Sulfur S 20
Formula: C
2119H
3212N
508O
545S
20
Total number of atoms: 6404
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 109360
Abs 0.1% (=1 g/l) 2.421, assuming all pairs of Cys residues form cystines
Ext. coefficient 108860
Abs 0.1% (=1 g/l) 2.410, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.15
This classifies the protein as stable.
Aliphatic index: 97.44
Grand average of hydropathicity (GRAVY): 0.038