ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKESAASVAV RPQLREVFER FPADAHRTIE DEIHEMMKCY AFDDAIFLAE LHYETDKSNN 

        70         80         90        100        110        120 
SESLLLYADC LYRANKKEEC YGLLSSVKLS GARLFYLLAR VSYDLNKIDD CRGALFEHDD 

       130        140        150        160        170        180 
GVIRKDILEE PRVASHANLL HAQMLCDESY MDLALESCQK SLDENILLWS AIITYLRFGG 

       190        200        210        220        230        240 
HDLAHTFEKH HRKSNGLYLD SPASSLKSET PSPNVPGPSS SSAASTAEPS RLESSVRRST 

       250        260        270 
RGTIASANRE TRNTTSNITP RQSTPGSTHE GYT 

References and documentation are available.

Number of amino acids: 273

Molecular weight: 30554.11

Theoretical pI: 5.68

Amino acid composition: 
Ala (A)  25	  9.2%
Arg (R)  19	  7.0%
Asn (N)  11	  4.0%
Asp (D)  17	  6.2%
Cys (C)   6	  2.2%
Gln (Q)   4	  1.5%
Glu (E)  23	  8.4%
Gly (G)  11	  4.0%
His (H)  11	  4.0%
Ile (I)  11	  4.0%
Leu (L)  30	 11.0%
Lys (K)  12	  4.4%
Met (M)   5	  1.8%
Phe (F)   8	  2.9%
Pro (P)  11	  4.0%
Ser (S)  33	 12.1%
Thr (T)  15	  5.5%
Trp (W)   1	  0.4%
Tyr (Y)  11	  4.0%
Val (V)   9	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1326
Hydrogen    H	      2088
Nitrogen    N	       380
Oxygen      O	       428
Sulfur      S	        11

Formula: C1326H2088N380O428S11
Total number of atoms: 4233

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22265
Abs 0.1% (=1 g/l)   0.729, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.716, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.77
This classifies the protein as unstable.



Aliphatic index: 77.29

Grand average of hydropathicity (GRAVY): -0.516