ProtParam
User-provided sequence:
10 20 30 40 50 60
MKEVVIVSAV RTAMGKFGGT LKDVPAVELG ATVIKEAINR AGIKPEIIDE VIMGNVIQAG
70 80 90 100 110 120
LGQSPGRQAA VKAGIPVEVP AFTLNKVCGS GLRAVGLAAQ MIKAGDADVV IAGGMENMSA
130 140 150 160 170 180
APYVLPNARW GQRMFDGKMV DTMVKDGLWE SFNDYHMGMT AENIAEKWEL TREMQDEFAC
190 200 210 220 230 240
ASQNKAEKAI KEGKFKDEIV PVVIKTRKGE VVFDTDEFPR FGATVESLAK LKPAFKKDGT
250 260 270 280 290 300
VTAGNASGIN DGAAALVIMS AEKAEELGLK PLAKIASYGS KGLDPAYMGY GPVGATKVAL
310 320 330 340 350 360
EKAGWKVEDL DLIEANEAFA SQSLAVAKDL GFDMEKVNVN GGAIALGHPV GCSGARILVT
370 380 390
LLHEMQRRDS KKGLATLCIG GGMGTALLVE R
References and
documentation are available.
Number of amino acids: 391
Molecular weight: 41228.77
Theoretical pI: 5.87
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1821
Hydrogen H 2959
Nitrogen N 495
Oxygen O 548
Sulfur S 21
Formula: C
1821H
2959N
495O
548S
21
Total number of atoms: 5844
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29700
Abs 0.1% (=1 g/l) 0.720, assuming all pairs of Cys residues form cystines
Ext. coefficient 29450
Abs 0.1% (=1 g/l) 0.714, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.85
This classifies the protein as stable.
Aliphatic index: 90.87
Grand average of hydropathicity (GRAVY): 0.028