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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKEVVIVSAV RTAMGKFGGT LKDVPAVELG ATVIKEAINR AGIKPEIIDE VIMGNVIQAG

70 80 90 100 110 120
LGQSPGRQAA VKAGIPVEVP AFTLNKVCGS GLRAVGLAAQ MIKAGDADVV IAGGMENMSA

130 140 150 160 170 180
APYVLPNARW GQRMFDGKMV DTMVKDGLWE SFNDYHMGMT AENIAEKWEL TREMQDEFAC

190 200 210 220 230 240
ASQNKAEKAI KEGKFKDEIV PVVIKTRKGE VVFDTDEFPR FGATVESLAK LKPAFKKDGT

250 260 270 280 290 300
VTAGNASGIN DGAAALVIMS AEKAEELGLK PLAKIASYGS KGLDPAYMGY GPVGATKVAL

310 320 330 340 350 360
EKAGWKVEDL DLIEANEAFA SQSLAVAKDL GFDMEKVNVN GGAIALGHPV GCSGARILVT

370 380 390
LLHEMQRRDS KKGLATLCIG GGMGTALLVE R


References and documentation are available.

Number of amino acids: 391 Molecular weight: 41228.77 Theoretical pI: 5.87
Amino acid composition: 
Ala (A) 52 13.3% Arg (R) 13 3.3% Asn (N) 13 3.3% Asp (D) 20 5.1% Cys (C) 4 1.0% Gln (Q) 9 2.3% Glu (E) 29 7.4% Gly (G) 45 11.5% His (H) 3 0.8% Ile (I) 22 5.6% Leu (L) 29 7.4% Lys (K) 33 8.4% Met (M) 17 4.3% Phe (F) 12 3.1% Pro (P) 14 3.6% Ser (S) 15 3.8% Thr (T) 16 4.1% Trp (W) 4 1.0% Tyr (Y) 5 1.3% Val (V) 36 9.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 49 Total number of positively charged residues (Arg + Lys): 46 Atomic composition: Carbon C 1821 Hydrogen H 2959 Nitrogen N 495 Oxygen O 548 Sulfur S 21 Formula: C1821H2959N495O548S21 Total number of atoms: 5844 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 29700 Abs 0.1% (=1 g/l) 0.720, assuming all pairs of Cys residues form cystines Ext. coefficient 29450 Abs 0.1% (=1 g/l) 0.714, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.85 This classifies the protein as stable. Aliphatic index: 90.87 Grand average of hydropathicity (GRAVY): 0.028