ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKEYWTSLAS LLGVLAFCQS LMNSVFPPEL RFAISKLFNK FFKLFSTFCY FDITEIDGVN 

        70         80         90        100        110        120 
TNELYNAVQL YLSSSVSIAG NRLSLTRAVN SSSVTFGLSN NDSIVDTFNS VTVVWEHIVT 

       130        140        150        160        170        180 
QRQTQTFAWR PMPEEKRGFT LRIKKKDKNL ILDSYLDYIM EKANEIRRLN QDRLLYTNSR 

       190        200        210        220        230        240 
GGSLDSRGLP WESVPFKHPS TFDTLAMDPV KKQQIMEDLK DFAECQSFYE RTGRAWKRGY 

       250        260        270        280        290        300 
LLYGPPGTGK SSMIAAMANY LRYDIYDLEL TEVKSNSELR KLLMKTSSKS IIVIEDIDCS 

       310        320        330        340        350        360 
INLTNRNKKQ STGSYNEPEM LTGSGLGDDL GDGNTITLSG LLNFTDGLWS CCGSERIFVF 

       370        380        390        400        410        420 
TTNHIEKLDP ALLRSGRMDM HIHMSYCTFS SVKILLRNYL GFEEGDLNDV VLKELAEVVD 

       430        440        450        460        470        480 
RAEITPADVS EALIKNRRDK ERAVRELLVD LRSRVERNEK NGKSRVQNVS LEEQENRAFD 

       490        500 
SLYAEENGGE EEEIEDNICK SSDDCS 

References and documentation are available.

Number of amino acids: 506

Molecular weight: 57615.18

Theoretical pI: 5.24

Amino acid composition: 
Ala (A)  22	  4.3%
Arg (R)  32	  6.3%
Asn (N)  32	  6.3%
Asp (D)  33	  6.5%
Cys (C)   9	  1.8%
Gln (Q)  12	  2.4%
Glu (E)  41	  8.1%
Gly (G)  28	  5.5%
His (H)   5	  1.0%
Ile (I)  27	  5.3%
Leu (L)  56	 11.1%
Lys (K)  30	  5.9%
Met (M)  13	  2.6%
Phe (F)  23	  4.5%
Pro (P)  13	  2.6%
Ser (S)  50	  9.9%
Thr (T)  29	  5.7%
Trp (W)   6	  1.2%
Tyr (Y)  17	  3.4%
Val (V)  28	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2536
Hydrogen    H	      4006
Nitrogen    N	       692
Oxygen      O	       795
Sulfur      S	        22

Formula: C2536H4006N692O795S22
Total number of atoms: 8051

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58830
Abs 0.1% (=1 g/l)   1.021, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   1.012, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.82
This classifies the protein as unstable.



Aliphatic index: 84.37

Grand average of hydropathicity (GRAVY): -0.408