ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKFCLYCCHC YIVICGKATH YYKSSKATSN LKSSNRVLMR NPMSPSEQHS QHNSTLAASP 

        70         80         90        100        110        120 
FVSNVSAART QQSLPTDAQN DRLQQPWNRT NTATSPYQSL ANSPLIQKLQ ANIMTPHQPS 

       130        140        150        160        170        180 
ANSNSNSNSI TGNVVNDNNL LASMSKNSMF GSTIPSTLRK VSLQREYKDS VDGVVRDEDN 

       190        200        210        220        230        240 
DEDVHNNGDA AANANNDRES KLGHNGPLTT TTLTTTTTAT QLDVSELSAI ERLRLWRFDA 

       250        260        270        280        290        300 
LMQHMYRTAE YIADKVYNIS NDPDDAFWLG QVYYNNNQYV RAVELITRNN LDGVNILCRY 

       310        320        330        340        350        360 
LLGLSFVKLQ RFDDALDVIG EYNPFSEDPS TTAANTMSNN GNNSNTSQPV TDGGIKMESS 

       370        380        390        400        410        420 
LCFLRGKIYF AQNNFNKARD AFREAILVDI KNFEAFEMLL SKNLLTPQEE WDLFDSLDFK 

       430        440        450        460        470        480 
EFGEDKEIMK NLYKINLSKY INTEDITKSN EILAKDYKLA DNVDVVRSKV DICYTQCKFN 

       490        500        510        520        530        540 
ECLELCETVL ENDEFNTNIL PAYIGCLYEL SNKNKLFLLS HRLAETFPKS AITWFSVATY 

       550        560        570        580        590        600 
YMSLDRISEA QKYYSKSSIL DPSFAAAWLG FAHTYALEGE QDQALTAYST ASRFFPGMHL 

       610        620        630        640        650        660 
PKLFLGMQFM AMNSLNLAES YFVLAYDICP NDPLVLNEMG VMYFKKNEFV KAKKYLKKAL 

       670        680        690        700        710        720 
EVVKDLDPSS RTTISIQLNL GHTYRKLNEN EIAIKCFRCV LEKNDKNSEI HCSLGYLYLK 

       730        740        750        760        770        780 
TKKLQKAIDH LHKSLYLKPN NSSATALLKN ALELNVTLSL DASHPLIDKS NLMSQASKDK 

       790        800        810        820        830        840 
ASLNKKRSSL TYDPVNMAKR LRTQKEIFDQ NNKALRKGGH DSKTGSNNAD DDFDADMELE 

References and documentation are available.

Number of amino acids: 840

Molecular weight: 94992.20

Theoretical pI: 6.32

Amino acid composition: 
Ala (A)  63	  7.5%
Arg (R)  31	  3.7%
Asn (N)  78	  9.3%
Asp (D)  55	  6.5%
Cys (C)  16	  1.9%
Gln (Q)  30	  3.6%
Glu (E)  44	  5.2%
Gly (G)  27	  3.2%
His (H)  17	  2.0%
Ile (I)  36	  4.3%
Leu (L)  93	 11.1%
Lys (K)  62	  7.4%
Met (M)  22	  2.6%
Phe (F)  34	  4.0%
Pro (P)  27	  3.2%
Ser (S)  77	  9.2%
Thr (T)  50	  6.0%
Trp (W)   6	  0.7%
Tyr (Y)  36	  4.3%
Val (V)  36	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      4179
Hydrogen    H	      6559
Nitrogen    N	      1143
Oxygen      O	      1310
Sulfur      S	        38

Formula: C4179H6559N1143O1310S38
Total number of atoms: 13229

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87640
Abs 0.1% (=1 g/l)   0.923, assuming all pairs of Cys residues form cystines


Ext. coefficient    86640
Abs 0.1% (=1 g/l)   0.912, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.53
This classifies the protein as stable.



Aliphatic index: 79.82

Grand average of hydropathicity (GRAVY): -0.483