ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKFGKYLEAR QLELAEYNSH FIDYKALKKL IKQLAIPTLK ASSDLDLHLT LDDIDEKIIH 

        70         80         90        100        110        120 
QRLQENKAAF FFKLERELEK VNGYYLARES DLRIKFNILH SKYKDYKING KLNSNQATSF 

       130        140        150        160        170        180 
KNLYAAFKKF QKDLRNLEQY VELNKTGFSK ALKKWDKRSQ SHDKDFYLAT VVSIQPIFTR 

       190        200        210        220        230        240 
DGPLKLNDET LHILLELNDI DNNNRRADIQ SSTFTNDDDD DNNTSNNNKH NNNNNNNNNN 

       250        260        270        280        290        300 
NNNNNNNNIL HNNYELTTSK ISENQLEHLF QASSSSLDME MEIENWYKEI LNIATVKDVQ 

       310        320        330        340        350        360 
RKHALLRNFR ETKIFTYLLQ NSSESFHKNV FSLLKECLTT LFLLLVASPL DDNSLHIFYK 

       370        380        390        400        410        420 
SNQDHIDLSY CDEDDQVFSR KNVFHEAASC PEKSRLFILD EALTTSKLSK ETVQKLLNAQ 

       430        440        450        460        470        480 
DIHSRVPLHY AAELGKLEFV HSLLITNLLE DVDPIDSDSK TPLVLAITNN HIDVVRDLLT 

       490        500        510        520        530        540 
IGGANASPIE KPILDYSKNV ISSTKVQFDP LNVACKFNNH DAAKLLLEIR SKQNADNAKN 

       550        560        570        580        590        600 
KSSQHLCQPL FKKNSTGLCT LHIVAKIGGD PQLIQLLIRY GADPNEIDGF NKWTPIFYAV 

       610        620        630        640        650        660 
RSGHSEVITE LLKHNARLDI EDDNGHSPLF YALWESHVDV LNALLQRPLN LPSAPLNEIN 

       670        680        690        700        710        720 
SQSSTQRLNT IDLTPNDDKF DLDIQDSIPD FALPPPIIPL RKYGHNFLEK KIFIKLKLRP 

       730        740        750        760        770        780 
GLESIKLTQD NGIIMSSSPG RITLSSNLPE IIPRNVILPV RSGEINNFCK DISETNDEED 

       790        800        810        820        830        840 
DDEISEDHDD GEIIFQVDSI DDFSMDFEIF PSFGTRIIAK TTAMPFLFKK VAINSIATMN 

       850        860        870        880        890        900 
LPLFDTRLNN IGSLTLDYQI IFPYPGNPLK IINYEPYWKS TGSDLMTSSK DGNFVTSSSL 

       910        920        930        940        950        960 
NGSFISVLVC ALNDETIVAA PKPYVEFKGT KILLNDLTKE QLEKVVDYDF GKIDGSFDEV 

       970        980        990       1000       1010       1020 
TLKQYLSSRV VPLRSLLEVI PGSAQLVIRV YFPTDKEIDT IPIKISPFIN INQFIDKLLL 

      1030       1040       1050       1060       1070       1080 
IIFEHERFLR HSGSGSMRQI VFSSCNWEAC SILNWKQPNF PVLLQMKNLL RDSTTGKFVG 

      1090       1100       1110       1120       1130       1140 
DTPNCLKELA VNPQKMSYLN TELINIHTMV QFAMNNNLLG VTLPYEVLKI CPSLARIIKQ 

      1150       1160       1170 
NGLLLIASVG ENDQIPADGG YSGIYYACEL LFENNIDM 

References and documentation are available.

Number of amino acids: 1178

Molecular weight: 134029.38

Theoretical pI: 5.53

Amino acid composition: 
Ala (A)  55	  4.7%
Arg (R)  38	  3.2%
Asn (N) 107	  9.1%
Asp (D)  88	  7.5%
Cys (C)  13	  1.1%
Gln (Q)  42	  3.6%
Glu (E)  67	  5.7%
Gly (G)  41	  3.5%
His (H)  30	  2.5%
Ile (I)  98	  8.3%
Leu (L) 147	 12.5%
Lys (K)  87	  7.4%
Met (M)  14	  1.2%
Phe (F)  59	  5.0%
Pro (P)  51	  4.3%
Ser (S)  94	  8.0%
Thr (T)  56	  4.8%
Trp (W)   7	  0.6%
Tyr (Y)  34	  2.9%
Val (V)  50	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 155
Total number of positively charged residues (Arg + Lys): 125

Atomic composition:

Carbon      C	      6006
Hydrogen    H	      9459
Nitrogen    N	      1595
Oxygen      O	      1822
Sulfur      S	        27

Formula: C6006H9459N1595O1822S27
Total number of atoms: 18909

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    89910
Abs 0.1% (=1 g/l)   0.671, assuming all pairs of Cys residues form cystines


Ext. coefficient    89160
Abs 0.1% (=1 g/l)   0.665, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.03
This classifies the protein as unstable.



Aliphatic index: 98.09

Grand average of hydropathicity (GRAVY): -0.340