ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKFIDELDIE RVNQTLNFET NDCKIVGSCD IFTTKAVASD RKLYKTIDQH LDTILQENEN 

        70         80         90        100        110        120 
YNATLQQQLA APETNQSPCS SPFYSNRRDS NSFWEQKRRI SFSEYNSNNN TNNSNGNSSN 

       130        140        150        160        170        180 
NNNYSGPNGS SPATFPKSAK LNDQNLKELV SNYDSGSMSS SSLDSSSKND ERIRRRSSSS 

       190        200        210        220        230        240 
ISSFKSGKSS NNNYSSGTAT NNVNKRRKSS INERPSNLSL GPFGPINEPS SRKIFAYLIA 

       250        260        270        280        290        300 
ILNASYPDHD FSSVEPTDFV KTSLKTFISK FENTLYSLGR QPEEWVWEVI NSHMTLSDCV 

       310        320        330        340        350        360 
LFQYSPSNSF LEDEPGYLWN LIGFLYNRKR KRVAYLYLIC SRLNSSTGEV EDALAKKPQG 

       370        380        390 
KLIIDDGSNE YEGEYDFTYD ENVIDDKSDQ EESLQ 

References and documentation are available.

Number of amino acids: 395

Molecular weight: 44732.88

Theoretical pI: 5.15

Amino acid composition: 
Ala (A)  14	  3.5%
Arg (R)  19	  4.8%
Asn (N)  44	 11.1%
Asp (D)  25	  6.3%
Cys (C)   5	  1.3%
Gln (Q)  14	  3.5%
Glu (E)  27	  6.8%
Gly (G)  16	  4.1%
His (H)   3	  0.8%
Ile (I)  21	  5.3%
Leu (L)  30	  7.6%
Lys (K)  24	  6.1%
Met (M)   3	  0.8%
Phe (F)  18	  4.6%
Pro (P)  16	  4.1%
Ser (S)  62	 15.7%
Thr (T)  19	  4.8%
Trp (W)   4	  1.0%
Tyr (Y)  18	  4.6%
Val (V)  13	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1942
Hydrogen    H	      2996
Nitrogen    N	       544
Oxygen      O	       657
Sulfur      S	         8

Formula: C1942H2996N544O657S8
Total number of atoms: 6147

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49070
Abs 0.1% (=1 g/l)   1.097, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   1.091, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.00
This classifies the protein as unstable.



Aliphatic index: 63.44

Grand average of hydropathicity (GRAVY): -0.857