ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR 

        70         80         90        100        110        120 
LVIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALAQYRE QLEGKTFCVR VKRRGKHEFS 

       130        140        150        160        170        180 
SIEVERYVGG GLNQHIESAR VKLTNPDVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV 

       190        200        210        220        230        240 
LSLISGGFDS GVSSYMLMRR GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV 

       250        260        270        280        290        300 
AINFEPVVGE ILEKVDDGQM GVVLKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN 

       310        320        330        340        350        360 
LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS PTVKAIKAKI 

       370        380        390        400        410        420 
EAEEENFDFS ILDKVVEEAN NVDIREIAQQ TQQEVVEVET VSGFGPNDVI LDIRSVDEQD 

       430        440        450        460        470        480 
DKPLKVEGVD VVSLPFYKLS TKFGDLDQSK TWLLWCERGV MSRLQALYLR EQGFANVKVY 


RP 

References and documentation are available.

Number of amino acids: 482

Molecular weight: 54830.94

Theoretical pI: 6.16

Amino acid composition: 
Ala (A)  24	  5.0%
Arg (R)  36	  7.5%
Asn (N)  17	  3.5%
Asp (D)  29	  6.0%
Cys (C)   5	  1.0%
Gln (Q)  20	  4.1%
Glu (E)  40	  8.3%
Gly (G)  34	  7.1%
His (H)  13	  2.7%
Ile (I)  36	  7.5%
Leu (L)  43	  8.9%
Lys (K)  27	  5.6%
Met (M)   9	  1.9%
Phe (F)  21	  4.4%
Pro (P)  13	  2.7%
Ser (S)  26	  5.4%
Thr (T)  20	  4.1%
Trp (W)   4	  0.8%
Tyr (Y)  13	  2.7%
Val (V)  52	 10.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2447
Hydrogen    H	      3908
Nitrogen    N	       684
Oxygen      O	       717
Sulfur      S	        14

Formula: C2447H3908N684O717S14
Total number of atoms: 7770

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41620
Abs 0.1% (=1 g/l)   0.759, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   0.755, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.80
This classifies the protein as unstable.



Aliphatic index: 100.19

Grand average of hydropathicity (GRAVY): -0.195