ProtParam
User-provided sequence:
10 20 30 40 50 60
MKFLLQQIRN MHVKPIKMRW LTGGVNYSYL LSTEDRRNSW LIDPAEPLEV SPKLSAEEKK
70 80 90 100 110 120
SIDAIVNTHH HYDHSGGNLA LYSILCQENS GHDIKIIGGS KSSPGVTEVP DNLQQYHLGN
130 140 150 160 170 180
LRVTCIRTPC HTKDSICYYI KDLETGEQCI FTGDTLFIAG CGRFFEGTGR DMDMALNQIM
190 200 210 220 230 240
LRAVGETNWN KVKIYPGHEY TKGNVSFIRA KIYSDIGQNK EFDALEQYCK SNECTTGHFT
250 260 270 280
LRDELGYNPF MRLDDRAVRL AVGDTAGTYP RSVVMQELRK LKNAM
References and
documentation are available.
Number of amino acids: 285
Molecular weight: 32338.83
Theoretical pI: 7.13
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1423
Hydrogen H 2234
Nitrogen N 400
Oxygen O 428
Sulfur S 17
Formula: C
1423H
2234N
400O
428S
17
Total number of atoms: 4502
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36370
Abs 0.1% (=1 g/l) 1.125, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 1.109, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.89
This classifies the protein as stable.
Aliphatic index: 79.02
Grand average of hydropathicity (GRAVY): -0.457