ProtParam
User-provided sequence:
10 20 30 40 50 60
MKFSAYLWWL FLNLALVKGT SLLSNVTLAE DSFWEHFQAY TNTKHLNQEW ITSEAVNNEG
70 80 90 100 110 120
SKIYGAQWRL SQGRLQGSAW DKGIAVRTGN AAAMIGHLLE TPINVSETDT LVVQYEIKLD
130 140 150 160 170 180
NSLTCGGAFI KLMSGFMNVE ALKHYAPDTE GVELVFGPDY CAPEINGVQF AINKVDKITH
190 200 210 220 230 240
ESKLRYLQEM PLSKLTDTSQ SHLYTLIIDE SAQSFQILID GKTVMVREHI EDKKKVNFEP
250 260 270 280 290 300
PITPPLMIPD VSVAKPHDWD DRIRIPDPEA VKLSDRDERD PLMIPHPDGT EPPEWNSSIP
310 320 330 340 350 360
EYILDPNAQK PSWWKELEHG EWIPPMIKNP LCTAERGCGQ QIPGLINNAK YKGPGELNEI
370 380 390 400 410 420
INPNYMGEWH PPEIENPLYY EEQHPLRIEN VISGVILEFW SGSPNMLISN IYVGKNVTEA
430 440 450 460 470 480
QIIGNKTWLM RDRAFRGSDG PTERKFMNSR LGNLQTTFHN ERESPNPFDR IIDRILEQPL
490 500
KFVLTAAVVL LTTSVLCCVV FT
References and
documentation are available.
Number of amino acids: 502
Molecular weight: 56967.94
Theoretical pI: 5.15
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2565
Hydrogen H 3969
Nitrogen N 675
Oxygen O 756
Sulfur S 19
Formula: C
2565H
3969N
675O
756S
19
Total number of atoms: 7984
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 98235
Abs 0.1% (=1 g/l) 1.724, assuming all pairs of Cys residues form cystines
Ext. coefficient 97860
Abs 0.1% (=1 g/l) 1.718, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.70
This classifies the protein as stable.
Aliphatic index: 88.17
Grand average of hydropathicity (GRAVY): -0.345