ProtParam
User-provided sequence:
10 20 30 40 50 60
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT
70 80 90 100 110 120
NNDLIMIPDL SRRDDIHTLL LGRNNIVEVD GRLLPMNVQN LTLSNNSIRR FEDLQRLRRA
130 140 150 160 170 180
PRTLKNLTLI GNQVCHLANY REHVLRLVPH LETLDFQNVT AEERKSAMSF PRQADGDTLG
190 200 210 220 230
PVNTAIRDNG SRDKTMEIMN LVVSKMTVER RNELKKQLAE ATSLEEIARL EKLLSGGV
References and
documentation are available.
Number of amino acids: 238
Molecular weight: 27193.21
Theoretical pI: 7.07
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1182
Hydrogen H 1950
Nitrogen N 350
Oxygen O 363
Sulfur S 10
Formula: C
1182H
1950N
350O
363S
10
Total number of atoms: 3855
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.224, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.219, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.95
This classifies the protein as unstable.
Aliphatic index: 100.71
Grand average of hydropathicity (GRAVY): -0.430