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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT

70 80 90 100 110 120
NNDLIMIPDL SRRDDIHTLL LGRNNIVEVD GRLLPMNVQN LTLSNNSIRR FEDLQRLRRA

130 140 150 160 170 180
PRTLKNLTLI GNQVCHLANY REHVLRLVPH LETLDFQNVT AEERKSAMSF PRQADGDTLG

190 200 210 220 230
PVNTAIRDNG SRDKTMEIMN LVVSKMTVER RNELKKQLAE ATSLEEIARL EKLLSGGV


References and documentation are available.

Number of amino acids: 238 Molecular weight: 27193.21 Theoretical pI: 7.07
Amino acid composition: 
Ala (A) 10 4.2% Arg (R) 20 8.4% Asn (N) 18 7.6% Asp (D) 17 7.1% Cys (C) 2 0.8% Gln (Q) 8 3.4% Glu (E) 15 6.3% Gly (G) 9 3.8% His (H) 7 2.9% Ile (I) 13 5.5% Leu (L) 34 14.3% Lys (K) 12 5.0% Met (M) 8 3.4% Phe (F) 5 2.1% Pro (P) 10 4.2% Ser (S) 14 5.9% Thr (T) 16 6.7% Trp (W) 0 0.0% Tyr (Y) 4 1.7% Val (V) 16 6.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 32 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 1182 Hydrogen H 1950 Nitrogen N 350 Oxygen O 363 Sulfur S 10 Formula: C1182H1950N350O363S10 Total number of atoms: 3855 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 6085 Abs 0.1% (=1 g/l) 0.224, assuming all pairs of Cys residues form cystines Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.219, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 43.95 This classifies the protein as unstable. Aliphatic index: 100.71 Grand average of hydropathicity (GRAVY): -0.430