ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKGDRSRQNM AVTKKAKLKK NDEPKKVLKT AATEKGEGSK RYSLWNFWIS TLFLKLLLIP 

        70         80         90        100        110        120 
DYFSTDFDVH RNWLAITNKL PISEWYYEHT SQWTLDYPPF FAYFEWFLSQ FVPKSVRDDG 

       130        140        150        160        170        180 
CLDIVEIGKF GLPTIVFQRL TVIFSEILLF VILQIYINTT KLSERSQSFV VASSIVLSPG 

       190        200        210        220        230        240 
FLIIDHIHFQ YNGFLFAILI GSIVAAKNKR YILCAVLYTT AICFKHIFLY LAPCYFVFLL 

       250        260        270        280        290        300 
RAYVLNVNNF KFKSYKDFLF LIRWANLLKL ATVVVGIFTI CFLPFAHQMP QVLSRLFPFS 

       310        320        330        340        350        360 
RGLTHAYWAP NFWALYSFMD KILTTVMLKL PYVHTFATKF IKPPLIPQNI KEINERLAAN 

       370        380        390        400        410        420 
NNGSKGLVQD VFFVILPQIP PKLTFILTIF YQVLAVLPLL FDPSFKRFVG SLTLCGLASF 

       430        440        450        460        470        480 
LFGWHVHEKA IMLVIIPFTF LVGFDRRLLV PFMLVASAGY VSLYPLLYKG QDFFIKTLYT 

       490        500        510        520        530        540 
YVWCIIYFAA FRKTTKISSS VERRIFFLDR LALTYIFSLL PIVTVLQILD EVKWRYSFLQ 

       550        560        570 
KFEFLGLMIY SVYCSLGIIS SWFALSWLYN FDELLWQ 

References and documentation are available.

Number of amino acids: 577

Molecular weight: 67385.05

Theoretical pI: 9.52

Amino acid composition: 
Ala (A)  32	  5.5%
Arg (R)  21	  3.6%
Asn (N)  19	  3.3%
Asp (D)  19	  3.3%
Cys (C)   8	  1.4%
Gln (Q)  17	  2.9%
Glu (E)  16	  2.8%
Gly (G)  21	  3.6%
His (H)  10	  1.7%
Ile (I)  48	  8.3%
Leu (L)  81	 14.0%
Lys (K)  38	  6.6%
Met (M)   8	  1.4%
Phe (F)  60	 10.4%
Pro (P)  26	  4.5%
Ser (S)  37	  6.4%
Thr (T)  31	  5.4%
Trp (W)  15	  2.6%
Tyr (Y)  29	  5.0%
Val (V)  41	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      3243
Hydrogen    H	      4895
Nitrogen    N	       749
Oxygen      O	       781
Sulfur      S	        16

Formula: C3243H4895N749O781S16
Total number of atoms: 9684

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   126210
Abs 0.1% (=1 g/l)   1.873, assuming all pairs of Cys residues form cystines


Ext. coefficient   125710
Abs 0.1% (=1 g/l)   1.866, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.78
This classifies the protein as stable.



Aliphatic index: 113.34

Grand average of hydropathicity (GRAVY): 0.487