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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST SGASPYMHSH 

        70         80         90        100        110        120 
HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN LGMYHSDTNT RSSRSIASTL 

       130        140        150        160        170        180 
KDDSPSVCSE DEISNSSSQK SNAQDETPIA YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT 

       190        200        210        220        230        240 
TLEKSLNTSG ISNSSGTNNN NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT 

       250        260        270        280        290        300 
VATNYNSSMT APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND 

       310        320        330        340        350        360 
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP ASNSSIANEN 

       370        380        390        400        410        420 
HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV YPHPDLRQDS DGKIISGTIE 

       430        440        450        460        470        480 
SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV INLLLMFPLL DHFIKFLKVF NQFGLSFTKN 

       490        500        510        520        530        540 
KSRLTNNSTQ FYNISPAVDD SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH 

       550        560        570        580        590        600 
NDEISNNLKI KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV 

       610        620        630        640        650        660 
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL LISHLFPTNP 

       670        680        690        700        710        720 
EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI ICSIPVLRSS SWKYVPDQSL 

       730        740        750        760        770        780 
KTIFKDWVPT IIQLERRQRT SKSTSSVFIL APPNLDDDFT RANVISYFGD LLIHADDLPS 

       790        800        810        820        830        840 
DTKFKYLEKK INRTKNAFHK WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS 

       850        860        870        880        890        900 
QPPLNIEGIM KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT 

       910        920        930        940        950        960 
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN DNNKVTGVGK 

       970        980        990       1000       1010       1020 
IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL VFKSVYDNDG KSRPASMVIE 

      1030       1040       1050       1060       1070       1080 
TPKRFSQHSS MLINNPATPN QKMRDSLDTT GRLSKTLENM DFFNNIGQVS DSLKESIIRV 

      1090       1100       1110       1120       1130       1140 
VLKSSSLEKI FDLLVLTSNI FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV 

      1150       1160       1170       1180       1190       1200 
KLTMDNDIFT ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE 

      1210       1220       1230       1240       1250       1260 
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH YSDFTDDLCN 

      1270       1280       1290       1300       1310       1320 
NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET ENLLTTLTDL FHGIKSAYQK 

      1330       1340       1350       1360       1370       1380 
QLYRPIGVNR TQKRITDILN SFNTFSFTDL NNIIDDPSFS DDMIRSFQKL HSTNYEDILE 

      1390       1400       1410       1420       1430       1440 
WIYQLDNFIS KKFNLVSKKD WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH 

      1450       1460       1470       1480       1490       1500 
EFEISNLFTW ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS 

      1510       1520       1530       1540       1550       1560 
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA HAIISPESRN 

      1570       1580       1590       1600       1610       1620 
YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF IEIDDVFSKN CKNLCPCPGW 

      1630       1640       1650       1660       1670       1680 
FISRLLEISQ FVPNMSITNS KLINFDKRRF VNNIISNVLD LIPNEREFPL DIEMSDENPS 

      1690       1700       1710       1720       1730       1740 
KRTTFGRILF NNFEDVNKVY RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK 

      1750       1760       1770       1780       1790       1800 
LEVLLDQEKI LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES 

      1810       1820       1830       1840       1850       1860 
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS SSNYSLNSIL 

      1870       1880       1890       1900       1910       1920 
SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI MEIINHRNIP AYYYAFKIVM 

      1930       1940       1950       1960       1970       1980 
QNGHEYLIQT ASSSDLTEWI KMIKASKRFS FHSKKYKGKT HNKIFGVPLE DVCERENTLI 

      1990       2000       2010       2020       2030       2040 
PTIVVKLLEE IELRGLDEVG LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA 

      2050       2060       2070       2080       2090       2100 
IAGCFKMYLR ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT 

      2110       2120       2130       2140       2150       2160 
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV QTILQDFIKN 


PNDYFKQ


References and documentation are available. 

Number of amino acids: 2167

Molecular weight: 245430.28

Theoretical pI: 8.53


Amino acid composition: 
Ala (A)  88	  4.1%
Arg (R)  89	  4.1%
Asn (N) 172	  7.9%
Asp (D) 132	  6.1%
Cys (C)  16	  0.7%
Gln (Q)  78	  3.6%
Glu (E) 107	  4.9%
Gly (G)  71	  3.3%
His (H)  63	  2.9%
Ile (I) 157	  7.2%
Leu (L) 205	  9.5%
Lys (K) 162	  7.5%
Met (M)  45	  2.1%
Phe (F) 105	  4.8%
Pro (P)  87	  4.0%
Ser (S) 279	 12.9%
Thr (T) 132	  6.1%
Trp (W)  19	  0.9%
Tyr (Y)  58	  2.7%
Val (V) 102	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%



Total number of negatively charged residues (Asp + Glu): 239
Total number of positively charged residues (Arg + Lys): 251

Atomic composition:

Carbon      C	     10862
Hydrogen    H	     17143
Nitrogen    N	      2991
Oxygen      O	      3365
Sulfur      S	        61

Formula: C10862H17143N2991O3365S61
Total number of atoms: 34422

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   191920
Abs 0.1% (=1 g/l)   0.782, assuming all pairs of Cys residues form cystines


Ext. coefficient   190920
Abs 0.1% (=1 g/l)   0.778, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.62
This classifies the protein as unstable.



Aliphatic index: 82.86

Grand average of hydropathicity (GRAVY): -0.475