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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKGSTLDKIS AFLGELIGTG ILVFLGCMGC VKTDLFPNNH LQIVLNFGFA VLIAIQCFGC

70 80 90 100 110 120
VSGAHLNPAV TVAAYIYEMV TLRMAFAYFA AQMLGAFIGY GLLMVLLPSP TLTVGAGLCV

130 140 150 160 170 180
TLPHTSVTTG QALGIEFVIT SILVIVCCGV WDPRNSKFHD SVGIRFGLAI ACLACAAGPF

190 200 210 220 230 240
TGGSMNPARS FAPALWNKHF ESNWIYWLAP LSSSAITAYA YKVVFRREVV EAEITSNEKL


RQLEDVQLS


References and documentation are available.

Number of amino acids: 249 Molecular weight: 26757.47 Theoretical pI: 6.93
Amino acid composition: 
Ala (A) 27 10.8% Arg (R) 7 2.8% Asn (N) 9 3.6% Asp (D) 5 2.0% Cys (C) 9 3.6% Gln (Q) 6 2.4% Glu (E) 9 3.6% Gly (G) 22 8.8% His (H) 5 2.0% Ile (I) 17 6.8% Leu (L) 29 11.6% Lys (K) 7 2.8% Met (M) 7 2.8% Phe (F) 16 6.4% Pro (P) 10 4.0% Ser (S) 16 6.4% Thr (T) 15 6.0% Trp (W) 4 1.6% Tyr (Y) 7 2.8% Val (V) 22 8.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14 Total number of positively charged residues (Arg + Lys): 14 Atomic composition: Carbon C 1227 Hydrogen H 1910 Nitrogen N 306 Oxygen O 331 Sulfur S 16 Formula: C1227H1910N306O331S16 Total number of atoms: 3790 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 32930 Abs 0.1% (=1 g/l) 1.231, assuming all pairs of Cys residues form cystines Ext. coefficient 32430 Abs 0.1% (=1 g/l) 1.212, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.56 This classifies the protein as stable. Aliphatic index: 108.51 Grand average of hydropathicity (GRAVY): 0.688