ProtParam
User-provided sequence:
10 20 30 40 50 60
MKGSTLDKIS AFLGELIGTG ILVFLGCMGC VKTDLFPNNH LQIVLNFGFA VLIAIQCFGC
70 80 90 100 110 120
VSGAHLNPAV TVAAYIYEMV TLRMAFAYFA AQMLGAFIGY GLLMVLLPSP TLTVGAGLCV
130 140 150 160 170 180
TLPHTSVTTG QALGIEFVIT SILVIVCCGV WDPRNSKFHD SVGIRFGLAI ACLACAAGPF
190 200 210 220 230 240
TGGSMNPARS FAPALWNKHF ESNWIYWLAP LSSSAITAYA YKVVFRREVV EAEITSNEKL
RQLEDVQLS
References and
documentation are available.
Number of amino acids: 249
Molecular weight: 26757.47
Theoretical pI: 6.93
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 14
Atomic composition:
Carbon C 1227
Hydrogen H 1910
Nitrogen N 306
Oxygen O 331
Sulfur S 16
Formula: C
1227H
1910N
306O
331S
16
Total number of atoms: 3790
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32930
Abs 0.1% (=1 g/l) 1.231, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 1.212, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.56
This classifies the protein as stable.
Aliphatic index: 108.51
Grand average of hydropathicity (GRAVY): 0.688