ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKIFNTIQSV LFAAFFLKQG NCLASNGSTA LMGEVDMQTP FPEWLTEFTN LTQWPGIDPP 

        70         80         90        100        110        120 
YIPLDYINLT EVPELDRYYP GQCPKISREQ CSFDCYNCID VDDVTSCFKL SQTFDDGPAP 

       130        140        150        160        170        180 
ATEALLKKLR QRTTFFVLGI NTVNYPDIYE HILERGHLIG THTWSHEFLP SLSNEEIVAQ 

       190        200        210        220        230        240 
IEWSIWAMNA TGKHFPKYFR PPYGAIDNRV RAIVKQFGLT VVLWDLDTFD WKLITNDDFR 

       250        260        270        280        290        300 
TEEEILMDIN TWKGKRKGLI LEHDGARRTV EVAIKINELI GSDQLTIAEC IGDTDYIERY 


D 

References and documentation are available.

Number of amino acids: 301

Molecular weight: 34642.37

Theoretical pI: 4.73

Amino acid composition: 
Ala (A)  15	  5.0%
Arg (R)  13	  4.3%
Asn (N)  14	  4.7%
Asp (D)  23	  7.6%
Cys (C)   7	  2.3%
Gln (Q)  11	  3.7%
Glu (E)  21	  7.0%
Gly (G)  17	  5.6%
His (H)   6	  2.0%
Ile (I)  26	  8.6%
Leu (L)  27	  9.0%
Lys (K)  14	  4.7%
Met (M)   5	  1.7%
Phe (F)  17	  5.6%
Pro (P)  16	  5.3%
Ser (S)  12	  4.0%
Thr (T)  24	  8.0%
Trp (W)   8	  2.7%
Tyr (Y)  11	  3.7%
Val (V)  14	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1571
Hydrogen    H	      2389
Nitrogen    N	       399
Oxygen      O	       462
Sulfur      S	        12

Formula: C1571H2389N399O462S12
Total number of atoms: 4833

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60765
Abs 0.1% (=1 g/l)   1.754, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.743, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.03
This classifies the protein as stable.



Aliphatic index: 87.14

Grand average of hydropathicity (GRAVY): -0.246