ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKIIIFVCFI LIFYLPIQKK HVNSQQQGID SDDGDAESFF NKSYSEHQSA LEQKIFRGYN 

        70         80         90        100        110        120 
PKNRPMKNAS FPTIVDVHWH IIHVSINQRE QTMTVHGHIY MRWFDEFLVW DPKDFNNIQY 

       130        140        150        160        170        180 
ARVKKWQVWQ PKIKVSNSAS GLGSAFDFST SAHVIIQMMG KDRAKVEMYP TFSIKVGCAF 

       190        200        210        220        230        240 
DFTDYPYDLN KCAINLFSTS TMADVQLQNL YAIPPTLSFG WEEQKMKRII SDFKIQNVSS 

       250        260        270        280        290        300 
SALYYTNGNI TSSAPITGFD LGSTWSMLAV TVSFVRHSPL FEAAVVTPCI VVASLISMTF 

       310        320        330        340        350        360 
FIDSLSSTFL LMMLHFYVQL IFLHDLVEKL PLSVSEIPFC IKLIGILMYT NGLTLVLHSS 

       370        380        390        400        410        420 
LLCGSYYKCP VPQQLNKIFV IEEYIPNTFK EKVQIKKEES NKSWRDLMKL IRPIIGFVLI 

       430 
ILLICMFFVC LLL 

References and documentation are available.

Number of amino acids: 433

Molecular weight: 49771.29

Theoretical pI: 8.43

Amino acid composition: 
Ala (A)  18	  4.2%
Arg (R)  10	  2.3%
Asn (N)  19	  4.4%
Asp (D)  19	  4.4%
Cys (C)   9	  2.1%
Gln (Q)  20	  4.6%
Glu (E)  16	  3.7%
Gly (G)  16	  3.7%
His (H)  12	  2.8%
Ile (I)  43	  9.9%
Leu (L)  40	  9.2%
Lys (K)  29	  6.7%
Met (M)  16	  3.7%
Phe (F)  32	  7.4%
Pro (P)  19	  4.4%
Ser (S)  40	  9.2%
Thr (T)  20	  4.6%
Trp (W)   8	  1.8%
Tyr (Y)  16	  3.7%
Val (V)  31	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      2299
Hydrogen    H	      3539
Nitrogen    N	       563
Oxygen      O	       619
Sulfur      S	        25

Formula: C2299H3539N563O619S25
Total number of atoms: 7045

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68340
Abs 0.1% (=1 g/l)   1.373, assuming all pairs of Cys residues form cystines


Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.363, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.91
This classifies the protein as stable.



Aliphatic index: 99.68

Grand average of hydropathicity (GRAVY): 0.195