ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKIISLSISI GIAIIAVLAS KTLFKTHPEA FGIKAISYSF KKSLCDHHHH HHHHHHHHHR 

        70         80         90        100        110        120 
HKPSDTKRKV SICDDFPKNI PPLDTDTTSY LCVDKNGCCN FTTVQSAVDA VGNFSQRRNV 

       130        140        150        160        170        180 
IWINSGMYYE KVVIPKTKPN ITLQGQGFDI TAIAWNDTAY SANGTFYCAT VQVFGSQFVA 

       190        200        210        220        230        240 
KNISFMNVAP IPKPGDVGAQ AVAIRIAGDE SAFVGCGFFG AQDTLHDDRG RHYFKDCYIQ 

       250        260        270        280        290        300 
GSIDFIFGNA KSLYQDCRII SMANQLSPGS KAVNGAVTAN GRSSKDENSG FSFVNCTIGG 

       310        320        330        340        350        360 
TGHVWLGRAW RPYSRVVFVS TTMTDVIAPE GWNNFNDPSR DATIFYGEYN CSGPGADMSK 

       370        380        390 
RAPYVQKLNE TQVALLINTS FIDGDQWLQF SDL 

References and documentation are available.

Number of amino acids: 393

Molecular weight: 43349.92

Theoretical pI: 8.22

Amino acid composition: 
Ala (A)  31	  7.9%
Arg (R)  14	  3.6%
Asn (N)  23	  5.9%
Asp (D)  26	  6.6%
Cys (C)  11	  2.8%
Gln (Q)  15	  3.8%
Glu (E)   7	  1.8%
Gly (G)  31	  7.9%
His (H)  18	  4.6%
Ile (I)  31	  7.9%
Leu (L)  16	  4.1%
Lys (K)  22	  5.6%
Met (M)   6	  1.5%
Phe (F)  24	  6.1%
Pro (P)  16	  4.1%
Ser (S)  33	  8.4%
Thr (T)  24	  6.1%
Trp (W)   6	  1.5%
Tyr (Y)  13	  3.3%
Val (V)  26	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1934
Hydrogen    H	      2943
Nitrogen    N	       537
Oxygen      O	       568
Sulfur      S	        17

Formula: C1934H2943N537O568S17
Total number of atoms: 5999

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52995
Abs 0.1% (=1 g/l)   1.222, assuming all pairs of Cys residues form cystines


Ext. coefficient    52370
Abs 0.1% (=1 g/l)   1.208, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.24
This classifies the protein as stable.



Aliphatic index: 73.72

Grand average of hydropathicity (GRAVY): -0.221