ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKIKTIKRSA DDYVPVKSTQ ESQMPRNLNP ELHPFERARE YTKALNATKL ERMFAKPFVG 

        70         80         90        100        110        120 
QLGYGHRDGV YAIAKNYGSL NKLATGSADG VIKYWNMSTR EEFVSFKAHY GLVTGLCVTQ 

       130        140        150        160        170        180 
PRFHDKKPDL KSQNFMLSCS DDKTVKLWSI NVDDYSNKNS SDNDSVTNEE GLIRTFDGES 

       190        200        210        220        230        240 
AFQGIDSHRE NSTFATGGAK IHLWDVNRLK PVSDLSWGAD NITSLKFNQN ETDILASTGS 

       250        260        270        280        290        300 
DNSIVLYDLR TNSPTQKIVQ TMRTNAICWN PMEAFNFVTA NEDHNAYYYD MRNLSRSLNV 

       310        320        330        340        350        360 
FKDHVSAVMD VDFSPTGDEI VTGSYDKSIR IYKTNHGHSR EIYHTKRMQH VFQVKYSMDS 

       370        380        390        400        410        420 
KYIISGSDDG NVRLWRSKAW ERSNVKTTRE KNKLEYDEKL KERFRHMPEI KRISRHRHVP 

       430        440        450        460        470        480 
QVIKKAQEIK NIELSSIKRR EANERRTRKD MPYISERKKQ IVGTVHKYED SGRDRKRRKE 


DDKRDTQEK 

References and documentation are available.

Number of amino acids: 489

Molecular weight: 56788.82

Theoretical pI: 9.42

Amino acid composition: 
Ala (A)  23	  4.7%
Arg (R)  38	  7.8%
Asn (N)  32	  6.5%
Asp (D)  37	  7.6%
Cys (C)   3	  0.6%
Gln (Q)  15	  3.1%
Glu (E)  30	  6.1%
Gly (G)  23	  4.7%
His (H)  16	  3.3%
Ile (I)  27	  5.5%
Leu (L)  26	  5.3%
Lys (K)  46	  9.4%
Met (M)  13	  2.7%
Phe (F)  17	  3.5%
Pro (P)  14	  2.9%
Ser (S)  43	  8.8%
Thr (T)  30	  6.1%
Trp (W)   7	  1.4%
Tyr (Y)  20	  4.1%
Val (V)  29	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      2482
Hydrogen    H	      3915
Nitrogen    N	       735
Oxygen      O	       764
Sulfur      S	        16

Formula: C2482H3915N735O764S16
Total number of atoms: 7912

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68425
Abs 0.1% (=1 g/l)   1.205, assuming all pairs of Cys residues form cystines


Ext. coefficient    68300
Abs 0.1% (=1 g/l)   1.203, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.14
This classifies the protein as unstable.



Aliphatic index: 64.17

Grand average of hydropathicity (GRAVY): -0.934