ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKINIRDSTM VRPATETPIT NLWNSNVDLV IPRFHTPSVY FYRPTGASNF FDPQVMKEAL 

        70         80         90        100        110        120 
SKALVPFYPM AGRLKRDDDG RIEIDCNGAG VLFVVADTPS VIDDFGDFAP TLNLRQLIPE 

       130        140        150        160        170        180 
VDHSAGIHSF PLLVLQVTFF KCGGASLGVG MQHHAADGFS GLHFINTWSD MARGLDLTIP 

       190        200        210        220        230        240 
PFIDRTLLRA RDPPQPAFHH VEYQPAPSMK IPLDPSKSGP ENTTVSIFKL TRDQLVALKA 

       250        260        270        280        290        300 
KSKEDGNTVS YSSYEMLAGH VWRSVGKARG LPNDQETKLY IATDGRSRLR PQLPPGYFGN 

       310        320        330        340        350        360 
VIFTATPLAV AGDLLSKPTW YAAGQIHDFL VRMDDNYLRS ALDYLEMQPD LSALVRGAHT 

       370        380        390        400        410        420 
YKCPNLGITS WVRLPIYDAD FGWGRPIFMG PGGIPYEGLS FVLPSPTNDG SLSVAIALQS 

       430 
EHMKLFEKFL FEI 

References and documentation are available.

Number of amino acids: 433

Molecular weight: 47974.92

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  32	  7.4%
Arg (R)  23	  5.3%
Asn (N)  15	  3.5%
Asp (D)  31	  7.2%
Cys (C)   3	  0.7%
Gln (Q)  12	  2.8%
Glu (E)  14	  3.2%
Gly (G)  33	  7.6%
His (H)  12	  2.8%
Ile (I)  23	  5.3%
Leu (L)  44	 10.2%
Lys (K)  17	  3.9%
Met (M)  12	  2.8%
Phe (F)  25	  5.8%
Pro (P)  36	  8.3%
Ser (S)  30	  6.9%
Thr (T)  24	  5.5%
Trp (W)   6	  1.4%
Tyr (Y)  14	  3.2%
Val (V)  27	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      2177
Hydrogen    H	      3348
Nitrogen    N	       576
Oxygen      O	       619
Sulfur      S	        15

Formula: C2177H3348N576O619S15
Total number of atoms: 6735

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53985
Abs 0.1% (=1 g/l)   1.125, assuming all pairs of Cys residues form cystines


Ext. coefficient    53860
Abs 0.1% (=1 g/l)   1.123, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.08
This classifies the protein as stable.



Aliphatic index: 85.82

Grand average of hydropathicity (GRAVY): -0.123