ProtParam
User-provided sequence:
10 20 30 40 50 60
MKIQIIWLTL VLIVVEANAV KQGKNATIPA LIVFGDSIMD TGNNNNLPTL LKCNFPPYGK
70 80 90 100 110 120
DYPGGFATGR FSDGRVPSDL IAEKIGLAKT LPAYMNPYLK PEDLLKGVTF ASGGTGYDPL
130 140 150 160 170 180
TAKIMSVISV WDQLIYFKEY ISKIKRHFGE EKAKDILEHS FFLVVSSSND LAHTYLAQAH
190 200 210 220 230 240
RYDRTSYANF LADSAVHFVR ELHKLGAQKI GVFSAVPVGC VPLQRTVFGD KELDGVILYI
250 260 270 280 290 300
NVYDTLFDMI QHPKKYGFEV ADRGCCGKGL LTISYLCNSL NQFTCSNSSA YIFWDSYHPS
310 320
KRAYQVIVDN LLDKYLSKVY
References and
documentation are available.
Number of amino acids: 320
Molecular weight: 35761.26
Theoretical pI: 8.36
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1639
Hydrogen H 2528
Nitrogen N 414
Oxygen O 461
Sulfur S 11
Formula: C
1639H
2528N
414O
461S
11
Total number of atoms: 5053
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45185
Abs 0.1% (=1 g/l) 1.264, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 1.253, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.92
This classifies the protein as stable.
Aliphatic index: 95.66
Grand average of hydropathicity (GRAVY): 0.003