ProtParam
User-provided sequence:
10 20 30 40 50 60
MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL
70 80 90 100 110 120
VDVTEQPHAN NKGLYKTRPN SSDKRVGVKR LYRPPKLTYV EDRLRSLFYK QHPWELSRPK
130 140 150 160 170 180
ILVENEIGDE NYDWSHMLQI GRPLDGESVI QRTMYLIKTK QYGDMVEAYD HARYEFYALR
190 200 210 220 230 240
MQEETEQQVA LEEAEMFGSL FGVSAIEHGI QKEQEVLDVW EKKVVEETEL MAARTSNPAG
250 260
SWKDDTTLNT AQEEESTTSE NLHF
References and
documentation are available.
Number of amino acids: 264
Molecular weight: 30513.38
Theoretical pI: 5.88
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1355
Hydrogen H 2117
Nitrogen N 373
Oxygen O 416
Sulfur S 7
Formula: C
1355H
2117N
373O
416S
7
Total number of atoms: 4268
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.487
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.99
This classifies the protein as unstable.
Aliphatic index: 74.20
Grand average of hydropathicity (GRAVY): -0.739