ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKISAALILS SLSSVAFSAP APAPADSHHE DHHKDEKPAV VTVTQYIDSN AATSTVESAA 

        70         80         90        100        110        120 
TTTTLSSSEK DTSEQKRDGG FQDGTVKCSD FPSVNGIVSL DWLGFGGWAS VMDMDANTSS 

       130        140        150        160        170        180 
ECKDGYYCSY ACEPGMSKTQ WPSDQPSDGK SVGGLYCKNG YLYRTNTDTS DLCSTDETSA 

       190        200        210        220        230        240 
KAINKKSDSI ALCRTDYPGS ENMVIPTVVD GGDSQPISVV DEDTYYQWQG KKTSAQYYIN 

       250        260        270        280        290        300 
NAGVSAEDGC IWGTSGSDVG NWAPLVLGAG STNGETYLSL IPNPNSNQAA NFNVKIVASD 

       310        320        330 
GANVQGSCAY EDGSFTGDGS DGCTVSVLSG SAEFVFY 

References and documentation are available.

Number of amino acids: 337

Molecular weight: 35412.47

Theoretical pI: 4.25

Amino acid composition: 
Ala (A)  29	  8.6%
Arg (R)   3	  0.9%
Asn (N)  17	  5.0%
Asp (D)  29	  8.6%
Cys (C)  10	  3.0%
Gln (Q)  11	  3.3%
Glu (E)  14	  4.2%
Gly (G)  32	  9.5%
His (H)   4	  1.2%
Ile (I)  12	  3.6%
Leu (L)  15	  4.5%
Lys (K)  16	  4.7%
Met (M)   5	  1.5%
Phe (F)   8	  2.4%
Pro (P)  14	  4.2%
Ser (S)  47	 13.9%
Thr (T)  26	  7.7%
Trp (W)   6	  1.8%
Tyr (Y)  15	  4.5%
Val (V)  24	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1523
Hydrogen    H	      2322
Nitrogen    N	       404
Oxygen      O	       540
Sulfur      S	        15

Formula: C1523H2322N404O540S15
Total number of atoms: 4804

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55975
Abs 0.1% (=1 g/l)   1.581, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   1.563, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.77
This classifies the protein as stable.



Aliphatic index: 60.50

Grand average of hydropathicity (GRAVY): -0.392