ProtParam
User-provided sequence:
10 20 30 40 50 60
MKITKYRTKK SLGQCFILDP AIAEKIVSYA GCLEQYNVIE VGPGLGIMTQ SILNKEVRRL
70 80 90 100 110 120
TAIEKDRRLS NIHSKLKEAH AEYDCIFEDI LDVNIEQLLS QSPLKMISNL PYNISVILLL
130 140 150 160 170 180
KLLPYIHRFE KLILMFQKEV ADRIVAQPNT KSYSILSILV QLLCDVRKVE DFPPEIFSPS
190 200 210 220 230 240
PKVYSSVIEI TPLLSPRFSV DNSYFAQVLK KLFHCRRKTI RNSLKSCIKD ADALFIGCNI
250 260
DPNARAESLT IEQLCSLTNA LKARNINII
References and
documentation are available.
Number of amino acids: 269
Molecular weight: 30615.04
Theoretical pI: 9.02
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1381
Hydrogen H 2257
Nitrogen N 365
Oxygen O 391
Sulfur S 12
Formula: C
1381H
2257N
365O
391S
12
Total number of atoms: 4406
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13910
Abs 0.1% (=1 g/l) 0.454, assuming all pairs of Cys residues form cystines
Ext. coefficient 13410
Abs 0.1% (=1 g/l) 0.438, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.65
This classifies the protein as unstable.
Aliphatic index: 115.99
Grand average of hydropathicity (GRAVY): 0.041