ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKKEKKTPTP LPSHHVLFAE PGFFLCNFFF VLLKHTQINP FFYFLFILLF IIYIAIIYFV 

        70         80         90        100        110        120 
FIRISHFSFS LCRQCNSLGR MIFMCAYLPA ASSRSVANPA LPPQKKKKKK KKGTLRTGEV 

       130 
EEQAKGNISF DLCGKQNFQ 

References and documentation are available.

Number of amino acids: 139

Molecular weight: 16094.23

Theoretical pI: 9.91

Amino acid composition: 
Ala (A)   8	  5.8%
Arg (R)   5	  3.6%
Asn (N)   6	  4.3%
Asp (D)   1	  0.7%
Cys (C)   5	  3.6%
Gln (Q)   6	  4.3%
Glu (E)   5	  3.6%
Gly (G)   6	  4.3%
His (H)   4	  2.9%
Ile (I)  11	  7.9%
Leu (L)  14	 10.1%
Lys (K)  15	 10.8%
Met (M)   3	  2.2%
Phe (F)  18	 12.9%
Pro (P)   9	  6.5%
Ser (S)   9	  6.5%
Thr (T)   5	  3.6%
Trp (W)   0	  0.0%
Tyr (Y)   4	  2.9%
Val (V)   5	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	       758
Hydrogen    H	      1165
Nitrogen    N	       189
Oxygen      O	       182
Sulfur      S	         8

Formula: C758H1165N189O182S8
Total number of atoms: 2302

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6210
Abs 0.1% (=1 g/l)   0.386, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.370, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.93
This classifies the protein as stable.



Aliphatic index: 86.33

Grand average of hydropathicity (GRAVY): 0.124