ProtParam
User-provided sequence:
10 20 30 40 50 60
MKKGAKRKGV SKAGRKAAVA ETQNDEVIEE TTKTTQEESQ QHEEEVVDEV KENGEEEEAK
70 80 90 100 110 120
GDQEEEEDAK PDSLEEDEEN QEDEVKAEEV KEEVEKKPVA RRGGKRKRAT KKDTEIKDEK
130 140 150 160 170 180
KPVPKAKKPR AAKVKEEPVY FEEKRSLEDL WKVAFPVGTE WDQLDALYEF NWDFQNLEEA
190 200 210 220 230 240
LEEGGKLYGK KVYVFGCTEP QLVPYKGANK IVHVPAVVVI ESPFPPSDKI GITSVQREVE
250 260 270 280 290 300
EIIPMKKMKM DWLPYIPIEK RDRQVDKMNS QIFTLGCTQR RSALRHMKED QLKKFEYCLP
310 320 330 340 350 360
YFYQPFKEDE LEQSTEVQIM FPSEPPVVCE FDWEFDELQE FVDKLVEEEA LPAEQADEFK
370 380 390 400 410 420
EYVKEQVRAA KKANREAKDA RKKAIEEMSE DTKQAFQKMK FYKFYPQPSP DTPDVSGVQS
430
PFINRYYGKA HEVL
References and
documentation are available.
Number of amino acids: 434
Molecular weight: 50516.84
Theoretical pI: 4.92
Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 74
Atomic composition:
Carbon C 2243
Hydrogen H 3508
Nitrogen N 590
Oxygen O 710
Sulfur S 13
Formula: C
2243H
3508N
590O
710S
13
Total number of atoms: 7064
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48610
Abs 0.1% (=1 g/l) 0.962, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 0.957, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 70.09
This classifies the protein as unstable.
Aliphatic index: 60.62
Grand average of hydropathicity (GRAVY): -1.034