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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKKKMIAIGF EGSANKIGVG IVTLDGTILA NPRHTYITPP GHGFLPRETA HHHLDHVLPL

70 80 90 100 110 120
VKSALETSQV TPEEIDCICY TKGPGMGAPL QVSAIVVRVL SQLWKKPIVA VNHCVAHIEM

130 140 150 160 170 180
GRVVTGADDP VVLYVSGGNT QVIAYSEGRY RIFGETIDIA VGNCLDRFAR VLKLSNDPSP

190 200 210 220 230 240
GYNIEQLAKK GENFIDLPYA VKGMDVSFSG ILSYIETTAE EKLKNNECTP ADLCYSLQET

250 260 270 280 290 300
VFAMLVEITE RAMAHCDKKD VLIVGGVGCN ERLQEMMRTM CSERDGKLFA TDDRYCIDNG

310 320 330 340 350
AMIAYTGLLA FVNGIETPIE DSTFTQRFRT DEVHAVWREK EAVLLGDKKV AAN


References and documentation are available.

Number of amino acids: 353 Molecular weight: 38767.62 Theoretical pI: 5.62
Amino acid composition: 
Ala (A) 28 7.9% Arg (R) 16 4.5% Asn (N) 14 4.0% Asp (D) 20 5.7% Cys (C) 10 2.8% Gln (Q) 8 2.3% Glu (E) 25 7.1% Gly (G) 29 8.2% His (H) 10 2.8% Ile (I) 25 7.1% Leu (L) 28 7.9% Lys (K) 20 5.7% Met (M) 11 3.1% Phe (F) 11 3.1% Pro (P) 15 4.2% Ser (S) 15 4.2% Thr (T) 23 6.5% Trp (W) 2 0.6% Tyr (Y) 11 3.1% Val (V) 32 9.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 45 Total number of positively charged residues (Arg + Lys): 36 Atomic composition: Carbon C 1714 Hydrogen H 2733 Nitrogen N 465 Oxygen O 515 Sulfur S 21 Formula: C1714H2733N465O515S21 Total number of atoms: 5448 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 28015 Abs 0.1% (=1 g/l) 0.723, assuming all pairs of Cys residues form cystines Ext. coefficient 27390 Abs 0.1% (=1 g/l) 0.707, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.51 This classifies the protein as stable. Aliphatic index: 92.78 Grand average of hydropathicity (GRAVY): -0.045