ProtParam
User-provided sequence:
10 20 30 40 50 60
MKKKMIAIGF EGSANKIGVG IVTLDGTILA NPRHTYITPP GHGFLPRETA HHHLDHVLPL
70 80 90 100 110 120
VKSALETSQV TPEEIDCICY TKGPGMGAPL QVSAIVVRVL SQLWKKPIVA VNHCVAHIEM
130 140 150 160 170 180
GRVVTGADDP VVLYVSGGNT QVIAYSEGRY RIFGETIDIA VGNCLDRFAR VLKLSNDPSP
190 200 210 220 230 240
GYNIEQLAKK GENFIDLPYA VKGMDVSFSG ILSYIETTAE EKLKNNECTP ADLCYSLQET
250 260 270 280 290 300
VFAMLVEITE RAMAHCDKKD VLIVGGVGCN ERLQEMMRTM CSERDGKLFA TDDRYCIDNG
310 320 330 340 350
AMIAYTGLLA FVNGIETPIE DSTFTQRFRT DEVHAVWREK EAVLLGDKKV AAN
References and
documentation are available.
Number of amino acids: 353
Molecular weight: 38767.62
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1714
Hydrogen H 2733
Nitrogen N 465
Oxygen O 515
Sulfur S 21
Formula: C
1714H
2733N
465O
515S
21
Total number of atoms: 5448
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28015
Abs 0.1% (=1 g/l) 0.723, assuming all pairs of Cys residues form cystines
Ext. coefficient 27390
Abs 0.1% (=1 g/l) 0.707, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.51
This classifies the protein as stable.
Aliphatic index: 92.78
Grand average of hydropathicity (GRAVY): -0.045