ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKKNNERVNT NPSLISKSYN MKKRPVIGTR RHFARKEIES DSDDEDDIFL AGERKKYLQN 

        70         80         90        100        110        120 
QSYERRKQKY VWSFNNITKS VIRNEHGNNL TLQEELLRHE AYEKNEEIAN ENDVDQGMFI 

       130        140        150        160        170        180 
QQNNILNMLV NLMKYFVHMD NISPVTTNGS LKESLRKYSS IIAVNIFSET GDLKKGIAIF 

       190        200        210        220        230        240 
QDLSDAEQAV QYLSNCKIDG RLISATITNH PKRLPNAEHL ESSTKTKDES QDKDKLTKLD 

       250        260        270        280        290        300 
RAKLEWLIQG LNCEKGSIGN LLCFAVEHVN NHVEITDAFL KEFFNDQPTD DTKVYDDDYI 

       310        320        330        340        350        360 
NERGEKKLSL IYLMNDILFN GISGTSLVWR YRFSFEPHVE RLLDDLYLFS KRLGGRIKED 

       370        380        390        400        410        420 
IFCKKVVKVI EVWKTWIAFQ EETLERAWRN FSGNTPQSPQ INSAALKVET KNSWTAISEE 

       430        440        450        460        470        480 
TEGLQDDEEY NGIPVDVNEL LNVEFISQIP MSTETSSSSS PQPTEERKAK FKPSFTQGTF 

       490 
VSKRMRMHAE DLF 

References and documentation are available.

Number of amino acids: 493

Molecular weight: 57108.29

Theoretical pI: 5.67

Amino acid composition: 
Ala (A)  20	  4.1%
Arg (R)  25	  5.1%
Asn (N)  39	  7.9%
Asp (D)  31	  6.3%
Cys (C)   4	  0.8%
Gln (Q)  20	  4.1%
Glu (E)  46	  9.3%
Gly (G)  20	  4.1%
His (H)  10	  2.0%
Ile (I)  34	  6.9%
Leu (L)  42	  8.5%
Lys (K)  42	  8.5%
Met (M)  10	  2.0%
Phe (F)  23	  4.7%
Pro (P)  14	  2.8%
Ser (S)  40	  8.1%
Thr (T)  27	  5.5%
Trp (W)   7	  1.4%
Tyr (Y)  14	  2.8%
Val (V)  25	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      2523
Hydrogen    H	      3968
Nitrogen    N	       696
Oxygen      O	       788
Sulfur      S	        14

Formula: C2523H3968N696O788S14
Total number of atoms: 7989

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59610
Abs 0.1% (=1 g/l)   1.044, assuming all pairs of Cys residues form cystines


Ext. coefficient    59360
Abs 0.1% (=1 g/l)   1.039, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.10
This classifies the protein as unstable.



Aliphatic index: 78.88

Grand average of hydropathicity (GRAVY): -0.696