ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKKQILIVAA QSILCSTVFG ERSNVGLSTE ELGGDSILYF NEDPIVIEID KKAIDKKTLE 

        70         80         90        100        110        120 
QLASTRDVVL TDLPDTLEFI DFNEYAKMKS KSDMLLEYIN EYEFDDFERS SEGGLEEEEE 

       130        140        150        160        170        180 
EDLIYDFNAQ AEDLGKLGSN IYEVVEEKNI VNTYDGNLIN ASTTESTTTI RPFVTSHSYV 

       190        200        210        220 
ASSTPYSNIS SLNEDYDNAS NFLTPTTVAL AVLLTILLFI QAY 

References and documentation are available.

Number of amino acids: 223

Molecular weight: 24988.77

Theoretical pI: 4.08

Amino acid composition: 
Ala (A)  13	  5.8%
Arg (R)   4	  1.8%
Asn (N)  14	  6.3%
Asp (D)  17	  7.6%
Cys (C)   1	  0.4%
Gln (Q)   5	  2.2%
Glu (E)  25	 11.2%
Gly (G)   9	  4.0%
His (H)   1	  0.4%
Ile (I)  18	  8.1%
Leu (L)  24	 10.8%
Lys (K)  11	  4.9%
Met (M)   3	  1.3%
Phe (F)  10	  4.5%
Pro (P)   5	  2.2%
Ser (S)  21	  9.4%
Thr (T)  18	  8.1%
Trp (W)   0	  0.0%
Tyr (Y)  11	  4.9%
Val (V)  13	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	      1111
Hydrogen    H	      1731
Nitrogen    N	       267
Oxygen      O	       377
Sulfur      S	         4

Formula: C1111H1731N267O377S4
Total number of atoms: 3490

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16390
Abs 0.1% (=1 g/l)   0.656, assuming all pairs of Cys residues form cystines


Ext. coefficient    16390
Abs 0.1% (=1 g/l)   0.656, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.65
This classifies the protein as unstable.



Aliphatic index: 96.19

Grand average of hydropathicity (GRAVY): -0.209