ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKKSPLPVQN VQSSDKNVAG KEALVKLLRW HFGHADFRGK QLEAIQAVVS GRDCFCLMPT 

        70         80         90        100        110        120 
GGGKSICYQI PALAKPGIVL VVSPLIALME NQVMALKEKG IAAEYLSSTQ ATHVKNKIHE 

       130        140        150        160        170        180 
DLDSGKPSVR LLYVTPELIA TKGFMLKLRK LHSRGLLNLI AIDEAHCISS WGHDFRPSYR 

       190        200        210        220        230        240 
QLSTLRDSLA DVPVLALTAT AAPKVQKDVI DSLNLRNPLV LKSSFNRPNI FYEVRYKDLL 

       250        260        270        280        290        300 
DNAYTDLGNL LKSCGNICAI IYCLERTTCD DLSVHLSSIG ISSAAYHAGL NSKMRSTVLD 

       310        320        330        340        350        360 
DWLSSKKQII VATVAFGMGI DKKDVRMVCH FNIPKSMESF YQESGRAGRD QLPSRSVLYY 

       370        380        390        400        410        420 
GVDDRKKMEY LLRNSENKKS SSSKKPTSDF EQIVTYCEGS GCRRKKILES FGEEFPVQQC 

       430        440        450        460        470        480 
KKTCDACKHP NQVAHCLEEL MTTASRRHNS SRIFITSSNN KTNEGQYSEF WNRNEDGSNS 

       490        500        510        520        530        540 
NEEISDSDDA TEAANSVTGP KLSKKLGLDE KLVLLEQAEE KYYERNKQVK KSEKNAISEA 

       550        560        570        580        590        600 
LRDSSKQRLL DALTRVLQLL ACVEEIDSQK GSEFLENECY RKYSKAGKSF YYSQIASTVR 

       610        620        630        640        650        660 
WLGTASRDEL MTRLSSVVSL AREQEPLEEP LLATEPVENI EEEDDGKTNT VESRVDEPTQ 

       670        680        690        700        710 
ELVVSPILSP IRLPQVPSFS EFVNRRKIKQ NTLIDKSSEG FDDKKPAKIM KLQ 

References and documentation are available.

Number of amino acids: 713

Molecular weight: 79944.17

Theoretical pI: 8.37

Amino acid composition: 
Ala (A)  45	  6.3%
Arg (R)  39	  5.5%
Asn (N)  33	  4.6%
Asp (D)  40	  5.6%
Cys (C)  17	  2.4%
Gln (Q)  28	  3.9%
Glu (E)  54	  7.6%
Gly (G)  34	  4.8%
His (H)  13	  1.8%
Ile (I)  36	  5.0%
Leu (L)  75	 10.5%
Lys (K)  61	  8.6%
Met (M)  13	  1.8%
Phe (F)  20	  2.8%
Pro (P)  28	  3.9%
Ser (S)  75	 10.5%
Thr (T)  31	  4.3%
Trp (W)   5	  0.7%
Tyr (Y)  21	  2.9%
Val (V)  45	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      3503
Hydrogen    H	      5655
Nitrogen    N	       983
Oxygen      O	      1090
Sulfur      S	        30

Formula: C3503H5655N983O1090S30
Total number of atoms: 11261

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59790
Abs 0.1% (=1 g/l)   0.748, assuming all pairs of Cys residues form cystines


Ext. coefficient    58790
Abs 0.1% (=1 g/l)   0.735, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.22
This classifies the protein as unstable.



Aliphatic index: 85.33

Grand average of hydropathicity (GRAVY): -0.462