ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS IQVTETPVRA 

        70         80         90        100        110        120 
GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI RSIAVHPTKP YVLSGSDDLT 

       130        140        150        160        170        180 
VKLWNWENNW ALEQTFEGHE HFVMCVAFNP KDPSTFASGC LDRTVKVWSL GQSTPNFTLT 

       190        200        210        220        230        240 
TGQERGVNYV DYYPLPDKPY MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL 

       250        260        270        280        290        300 
PIIISGSEDG TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL 

       310        320        330        340        350        360 
GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK ELGSVDVFPQ 

       370        380        390        400        410        420 
SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW GPDSNSYALI DETGQIKYYK 

       430        440        450        460        470        480 
NFKEVTSWSV PMHSAIDRLF SGALLGVKSD GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG 

       490        500        510        520        530        540 
ELVMIVNTNS NGDEASGYTL LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK 

       550        560        570        580        590        600 
WVGDVFIFTT ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS 

       610        620        630        640        650        660 
LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN ISPDQDQKFE 

       670        680        690        700        710        720 
LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA VEAFTNAHDL ESLFLLHSSF 

       730        740        750        760        770        780 
NNKEGLVTLA KDAERAGKFN LAFNAYWIAG DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD 

       790        800        810        820        830        840 
AVNDIVTKWK ENLILNGKNT VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE 

       850        860        870        880 
AEVEDSEFKE SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES 

References and documentation are available.

Number of amino acids: 889

Molecular weight: 99444.79

Theoretical pI: 4.63

Amino acid composition: 
Ala (A)  63	  7.1%
Arg (R)  30	  3.4%
Asn (N)  52	  5.8%
Asp (D)  61	  6.9%
Cys (C)   6	  0.7%
Gln (Q)  28	  3.1%
Glu (E)  77	  8.7%
Gly (G)  63	  7.1%
His (H)  14	  1.6%
Ile (I)  41	  4.6%
Leu (L)  78	  8.8%
Lys (K)  51	  5.7%
Met (M)   8	  0.9%
Phe (F)  43	  4.8%
Pro (P)  32	  3.6%
Ser (S)  66	  7.4%
Thr (T)  55	  6.2%
Trp (W)  20	  2.2%
Tyr (Y)  33	  3.7%
Val (V)  68	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 138
Total number of positively charged residues (Arg + Lys): 81

Atomic composition:

Carbon      C	      4456
Hydrogen    H	      6802
Nitrogen    N	      1158
Oxygen      O	      1400
Sulfur      S	        14

Formula: C4456H6802N1158O1400S14
Total number of atoms: 13830

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   159545
Abs 0.1% (=1 g/l)   1.604, assuming all pairs of Cys residues form cystines


Ext. coefficient   159170
Abs 0.1% (=1 g/l)   1.601, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.97
This classifies the protein as stable.



Aliphatic index: 81.47

Grand average of hydropathicity (GRAVY): -0.382