ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI LNKLGNKCKH 

        70         80         90        100        110        120 
ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK KNPYYDLLNP SIPIVADPPV 

       130        140        150        160        170        180 
QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF 

       190        200        210        220        230        240 
GISYDMANNS QTSAEPMDSK VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR 

       250        260        270        280        290        300 
ETSRMGHVPA MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF 

       310        320        330        340        350        360 
GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF SPNERWSCQR 


ILQELEKP 

References and documentation are available.

Number of amino acids: 368

Molecular weight: 42186.43

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  15	  4.1%
Arg (R)  16	  4.3%
Asn (N)  20	  5.4%
Asp (D)  31	  8.4%
Cys (C)   7	  1.9%
Gln (Q)  16	  4.3%
Glu (E)  19	  5.2%
Gly (G)  17	  4.6%
His (H)   8	  2.2%
Ile (I)  33	  9.0%
Leu (L)  31	  8.4%
Lys (K)  26	  7.1%
Met (M)  14	  3.8%
Phe (F)  17	  4.6%
Pro (P)  18	  4.9%
Ser (S)  28	  7.6%
Thr (T)  13	  3.5%
Trp (W)   4	  1.1%
Tyr (Y)  13	  3.5%
Val (V)  22	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1883
Hydrogen    H	      2954
Nitrogen    N	       498
Oxygen      O	       559
Sulfur      S	        21

Formula: C1883H2954N498O559S21
Total number of atoms: 5915

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41745
Abs 0.1% (=1 g/l)   0.990, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   0.981, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.93
This classifies the protein as stable.



Aliphatic index: 89.24

Grand average of hydropathicity (GRAVY): -0.299