ProtParam
User-provided sequence:
10 20 30 40 50 60
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL VVALWAVNVA
70 80 90 100 110 120
KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL QRIIAPPVIE NPKFVLYVGV
130 140 150 160 170 180
AGLISNTVGL FLFHDNDQEH GHGHGHSHGG IFADHEMHMP SSHTHTHAHV DGIENTTPMD
190 200 210 220 230 240
STDNISEIMP NAIVDSFMNE NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN
250 260 270 280 290 300
MHGVFLHVLG DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS
310 320 330 340 350 360
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL DISPEQFTDL
370 380 390 400 410 420
AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH LQNDPLSLRP KTYGTGISGS
430
TCLIDDAANC NTADCLEDH
References and
documentation are available.
Number of amino acids: 439
Molecular weight: 48154.87
Theoretical pI: 5.81
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 2159
Hydrogen H 3376
Nitrogen N 576
Oxygen O 644
Sulfur S 14
Formula: C
2159H
3376N
576O
644S
14
Total number of atoms: 6769
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44140
Abs 0.1% (=1 g/l) 0.917, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 0.911, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.45
This classifies the protein as stable.
Aliphatic index: 102.21
Grand average of hydropathicity (GRAVY): 0.056