ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLKLIVNGC EAPDDYKLLR TTINTVASLR KTAILRFNSE RLTIISTPKS SLNSSNNGTI 

        70         80         90        100        110        120 
LRGDTGQLWC TIPHDVFRLY TVISARELNT ITMECNCDSL LSVFKRYDRV MNQGSSSNMT 

       130        140        150        160        170        180 
IKLQSMPEWN TNNGTLSGGT AGGVDTTSKP NPICALGITF EEIVHTSGPN DAIVMNGGVD 

       190        200        210        220        230        240 
EHNGLPTTVG TGNLLASNKV IMHSFKVPVK LLFRAQDTRI QEPMINYIQL MMYKLPPISG 

       250        260        270        280        290        300 
EFGSAFHGFI RRVERYSNVN HIHLMGVKKK EHGNEGDDVE LKIIVNELDW HLEICWNGPL 

       310        320        330        340        350        360 
DSVIQRQEGL TDNPSQNQHI DTDGRQEEGS LPIIEADKPM SSLYTNTRDR EMEENIRYDE 

       370        380        390        400        410        420 
DLLRIEDSSI ADTRGNIYTA DTSGDTEFND ISVMVEKAEQ ESSSTHEVII RCKDWKVCSK 

       430        440        450        460        470 
LYAAFEEVVL AISHDESCVF HCSLDRGSLE DSEDVEKPRE RGQIIYYIAR SKGL 

References and documentation are available.

Number of amino acids: 474

Molecular weight: 53145.93

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  18	  3.8%
Arg (R)  26	  5.5%
Asn (N)  31	  6.5%
Asp (D)  31	  6.5%
Cys (C)  10	  2.1%
Gln (Q)  13	  2.7%
Glu (E)  37	  7.8%
Gly (G)  32	  6.8%
His (H)  13	  2.7%
Ile (I)  39	  8.2%
Leu (L)  39	  8.2%
Lys (K)  23	  4.9%
Met (M)  14	  3.0%
Phe (F)  12	  2.5%
Pro (P)  17	  3.6%
Ser (S)  41	  8.6%
Thr (T)  33	  7.0%
Trp (W)   5	  1.1%
Tyr (Y)  12	  2.5%
Val (V)  28	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2307
Hydrogen    H	      3678
Nitrogen    N	       650
Oxygen      O	       741
Sulfur      S	        24

Formula: C2307H3678N650O741S24
Total number of atoms: 7400

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46005
Abs 0.1% (=1 g/l)   0.866, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   0.854, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.44
This classifies the protein as stable.



Aliphatic index: 85.11

Grand average of hydropathicity (GRAVY): -0.416