ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH ILEKDPSLFP 

        70         80         90        100        110        120 
ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA AESIKARVVS LYGGAFPDYK 

       130        140        150        160        170        180 
ILFEDFKDPA KCAEIAKCGL SKRKMIYLES LAVYFTEKYK DIEKLFGQKD NDEEVIESLV 

       190        200        210        220        230        240 
TNVKGIGPWS AKMFLISGLK RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS 

       250        260        270        280        290 
KIKHKKYNWK IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS 

References and documentation are available.

Number of amino acids: 296

Molecular weight: 34332.90

Theoretical pI: 8.11

Amino acid composition: 
Ala (A)  21	  7.1%
Arg (R)  12	  4.1%
Asn (N)   9	  3.0%
Asp (D)  17	  5.7%
Cys (C)   4	  1.4%
Gln (Q)   5	  1.7%
Glu (E)  31	 10.5%
Gly (G)  11	  3.7%
His (H)   3	  1.0%
Ile (I)  21	  7.1%
Leu (L)  28	  9.5%
Lys (K)  38	 12.8%
Met (M)  10	  3.4%
Phe (F)  16	  5.4%
Pro (P)  11	  3.7%
Ser (S)  20	  6.8%
Thr (T)   9	  3.0%
Trp (W)   3	  1.0%
Tyr (Y)  13	  4.4%
Val (V)  14	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1558
Hydrogen    H	      2463
Nitrogen    N	       393
Oxygen      O	       449
Sulfur      S	        14

Formula: C1558H2463N393O449S14
Total number of atoms: 4877

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36120
Abs 0.1% (=1 g/l)   1.052, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   1.045, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.01
This classifies the protein as stable.



Aliphatic index: 85.37

Grand average of hydropathicity (GRAVY): -0.410