ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLNASTMLG QGKNIIQAEI DAACELIDFF RFNAVFAMEL EHYEPISTKI TKNTMQFRGM 

        70         80         90        100        110        120 
EGFVAAIAPF NFTAIGGNLP TAPALMGNVS LWKPSNTAVL SNYIIYELLE EAGMPPGILS 

       130        140        150        160        170        180 
FLPSDGPVFG DVITASPHLS AVNFTGSVPT FKTIWRKVAE NLDNYVTFPK LIGECGGKNF 

       190        200        210        220        230        240 
HFVHPSAHVD AVAAGTARSA WEYSGQKCSA CSRMYAPKSI WPKILEKISA IHKEIKLGDV 

       250        260        270        280        290        300 
RDGSVFLSAV IDDKAFARLK AYIDFAKTGA DGANVVLGGK CDDKTGYFIE PTLITVTDPK 

       310        320        330        340        350        360 
SKLLTEEMFG PVVTVLVYED SKVDEVLATV KDATPYGLTG AVFSQDKEFL YRARDVLRDA 

       370        380        390        400        410        420 
VGNMYLNDKS TGSIVGQQPF GGSRLSGTND KAGGPHYGLR WTSPLTIKET SVSLTDWKYP 


SME 

References and documentation are available.

Number of amino acids: 423

Molecular weight: 45994.64

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  39	  9.2%
Arg (R)  12	  2.8%
Asn (N)  17	  4.0%
Asp (D)  24	  5.7%
Cys (C)   5	  1.2%
Gln (Q)   7	  1.7%
Glu (E)  22	  5.2%
Gly (G)  36	  8.5%
His (H)   7	  1.7%
Ile (I)  25	  5.9%
Leu (L)  33	  7.8%
Lys (K)  29	  6.9%
Met (M)  11	  2.6%
Phe (F)  23	  5.4%
Pro (P)  23	  5.4%
Ser (S)  30	  7.1%
Thr (T)  29	  6.9%
Trp (W)   6	  1.4%
Tyr (Y)  14	  3.3%
Val (V)  31	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2079
Hydrogen    H	      3226
Nitrogen    N	       532
Oxygen      O	       613
Sulfur      S	        16

Formula: C2079H3226N532O613S16
Total number of atoms: 6466

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54110
Abs 0.1% (=1 g/l)   1.176, assuming all pairs of Cys residues form cystines


Ext. coefficient    53860
Abs 0.1% (=1 g/l)   1.171, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.44
This classifies the protein as stable.



Aliphatic index: 83.95

Grand average of hydropathicity (GRAVY): -0.041