ProtParam
User-provided sequence:
10 20 30 40 50 60
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG
70 80 90 100 110 120
FPMKQGVLLP TRIKLLLTKN VSCYRPRRDG ERKRKSVRGA IVGPDLAVLA LVIVKKGEQE
130 140 150 160 170 180
LEGLTDTTVP KRLGPKRANN IRKFFGLSKE DDVRDFVIRR EVTKGEKTYT KAPKIQRLVT
190 200 210 220 230
PQRLQRKRHQ RALKVRNAQA QREAAAEYAQ LLAKRLSERK AEKAEIRKRR ASSLKA
References and
documentation are available.
Number of amino acids: 236
Molecular weight: 26996.33
Theoretical pI: 10.44
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 1190
Hydrogen H 1987
Nitrogen N 371
Oxygen O 338
Sulfur S 3
Formula: C
1190H
1987N
371O
338S
3
Total number of atoms: 3889
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.276, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.276, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.36
This classifies the protein as stable.
Aliphatic index: 82.63
Grand average of hydropathicity (GRAVY): -0.771