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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG

70 80 90 100 110 120
FPMKQGVLLP TRIKLLLTKN VSCYRPRRDG ERKRKSVRGA IVGPDLAVLA LVIVKKGEQE

130 140 150 160 170 180
LEGLTDTTVP KRLGPKRANN IRKFFGLSKE DDVRDFVIRR EVTKGEKTYT KAPKIQRLVT

190 200 210 220 230
PQRLQRKRHQ RALKVRNAQA QREAAAEYAQ LLAKRLSERK AEKAEIRKRR ASSLKA


References and documentation are available.

Number of amino acids: 236 Molecular weight: 26996.33 Theoretical pI: 10.44
Amino acid composition: 
Ala (A) 18 7.6% Arg (R) 28 11.9% Asn (N) 7 3.0% Asp (D) 12 5.1% Cys (C) 1 0.4% Gln (Q) 12 5.1% Glu (E) 17 7.2% Gly (G) 17 7.2% His (H) 2 0.8% Ile (I) 12 5.1% Leu (L) 20 8.5% Lys (K) 28 11.9% Met (M) 2 0.8% Phe (F) 9 3.8% Pro (P) 9 3.8% Ser (S) 9 3.8% Thr (T) 10 4.2% Trp (W) 0 0.0% Tyr (Y) 5 2.1% Val (V) 18 7.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 29 Total number of positively charged residues (Arg + Lys): 56 Atomic composition: Carbon C 1190 Hydrogen H 1987 Nitrogen N 371 Oxygen O 338 Sulfur S 3 Formula: C1190H1987N371O338S3 Total number of atoms: 3889 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 7450 Abs 0.1% (=1 g/l) 0.276, assuming all pairs of Cys residues form cystines Ext. coefficient 7450 Abs 0.1% (=1 g/l) 0.276, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.36 This classifies the protein as stable. Aliphatic index: 82.63 Grand average of hydropathicity (GRAVY): -0.771