ProtParam
User-provided sequence:
10 20 30 40 50 60
MKLPIYLDYS ATTPVDPRVA EKMMQFLTLD GTFGNPASRS HRFGWQAEEA VDIARNQIAE
70 80 90 100 110 120
LVGADPREIV FTSGATESDN LAIKGAANFY QKKGKHIITS KTEHKAVLDT CRQLEREGFE
130 140 150 160 170 180
VTYLAPQRNG IIDLNELEAA MRDDTILVSI MHVNNEIGVV QDIATIGEMC RARGIIYHVD
190 200 210 220 230 240
ATQSVGKLPI DLSQLKVDLM SFSGHKIYGP KGIGALYVRR KPRIRIEAQM HGGGHERGMR
250 260 270 280 290 300
SGTLPVHQIV GMGEAYRIAK EEMETEMARL RGLRNRLWNG IKDIEEVYLN GDLEQGAPNI
310 320 330 340 350 360
LNVSFNYVEG ESLIMALKDL AVSSGSACTS ATLEPSYVLR ALGMNDELAH SSIRFSLGRF
370 380 390 400
TTEEEIDYTI DLVRKSIGRL RDLSPLWEMY KQGVDLNSIE WAHH
References and
documentation are available.
Number of amino acids: 404
Molecular weight: 45106.47
Theoretical pI: 5.80
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1983
Hydrogen H 3169
Nitrogen N 563
Oxygen O 602
Sulfur S 18
Formula: C
1983H
3169N
563O
602S
18
Total number of atoms: 6335
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41495
Abs 0.1% (=1 g/l) 0.920, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.917, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.61
This classifies the protein as stable.
Aliphatic index: 91.76
Grand average of hydropathicity (GRAVY): -0.274