ProtParam
User-provided sequence:
10 20 30 40 50 60
MKLQFFSFIT LFACLFTTAI FAKEDTAEDP EITHKVYFDI NHGDKQIGRI VMGLYGLTTP
70 80 90 100 110 120
QTVENFYQLT ISRDPKMGYL NSIFHRVIPN FMIQGGDFTH RSGIGGKSIF GNTFKDENFD
130 140 150 160 170 180
VKHDKPGRLS MANRGKNTNG SQFFITTVPC PWLDGKHVVF GEVLDGMDVV HYIENVKTDS
190 200 210 220
RNMPVKEVII VESGELETVP LDNKDAAKLQ EEIKAEASEA AHDEL
References and
documentation are available.
Number of amino acids: 225
Molecular weight: 25326.77
Theoretical pI: 5.35
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1137
Hydrogen H 1754
Nitrogen N 298
Oxygen O 340
Sulfur S 9
Formula: C
1137H
1754N
298O
340S
9
Total number of atoms: 3538
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13075
Abs 0.1% (=1 g/l) 0.516, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.511, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.21
This classifies the protein as stable.
Aliphatic index: 79.24
Grand average of hydropathicity (GRAVY): -0.283