ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG IKGIQIYIPT 

        70         80         90        100        110        120 
QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM SLTVLSKLIK SYNIDTNKIG 

       130        140        150        160        170        180 
RLEVGTETLI DKSKSVKSVL MQLFGENTDV EGIDTLNACY GGTNALFNSL NWIESNAWDG 

       190        200        210        220        230        240 
RDAIVVCGDI AIYDKGAARP TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS 

       250        260        270        280        290        300 
EYPYVDGHFS LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC 

       310        320        330        340        350        360 
KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV AKPFHKERVA 

       370        380        390        400        410        420 
QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL FSYGSGLAAS LYSCKIVGDV 

       430        440        450        460        470        480 
QHIIKELDIT NKLAKRITET PKDYEAAIEL RENAHLKKNF KPQGSIEHLQ SGVYYLTNID 

       490 
DKFRRSYDVK K 

References and documentation are available.

Number of amino acids: 491

Molecular weight: 55013.53

Theoretical pI: 8.36

Amino acid composition: 
Ala (A)  32	  6.5%
Arg (R)  17	  3.5%
Asn (N)  27	  5.5%
Asp (D)  33	  6.7%
Cys (C)   8	  1.6%
Gln (Q)  21	  4.3%
Glu (E)  23	  4.7%
Gly (G)  34	  6.9%
His (H)   8	  1.6%
Ile (I)  29	  5.9%
Leu (L)  42	  8.6%
Lys (K)  43	  8.8%
Met (M)   8	  1.6%
Phe (F)  18	  3.7%
Pro (P)  16	  3.3%
Ser (S)  35	  7.1%
Thr (T)  31	  6.3%
Trp (W)   4	  0.8%
Tyr (Y)  28	  5.7%
Val (V)  34	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2459
Hydrogen    H	      3853
Nitrogen    N	       653
Oxygen      O	       746
Sulfur      S	        16

Formula: C2459H3853N653O746S16
Total number of atoms: 7727

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64220
Abs 0.1% (=1 g/l)   1.167, assuming all pairs of Cys residues form cystines


Ext. coefficient    63720
Abs 0.1% (=1 g/l)   1.158, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.09
This classifies the protein as stable.



Aliphatic index: 82.99

Grand average of hydropathicity (GRAVY): -0.380