ProtParam
User-provided sequence:
10 20 30 40 50 60
MKLYFIALQF LTVVPLPFRL RWDERALGRS MAFFPLAGLT LGTLLAGADM LFAALVPRPV
70 80 90 100 110 120
SDLLLVALLA GVTGALHLDG LADVCDGLAA RGGRERFLAV MKDPRVGAVG VVGLVLGLAL
130 140 150 160 170 180
KYQALLAVPD DLKRQALLLF PAVARYAQVQ MTVGANRARQ DGLGAAFIAG TGMFQLVTAG
190 200 210 220 230 240
TITAVAAWLL LGPAGMAVLA ASVLFTGGFR WWFHRHLGGV TGDVIGCASE LNEILCLLVI
LALHRG
References and
documentation are available.
Number of amino acids: 246
Molecular weight: 25927.93
Theoretical pI: 9.50
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 1194
Hydrogen H 1919
Nitrogen N 319
Oxygen O 304
Sulfur S 10
Formula: C
1194H
1919N
319O
304S
10
Total number of atoms: 3746
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26595
Abs 0.1% (=1 g/l) 1.026, assuming all pairs of Cys residues form cystines
Ext. coefficient 26470
Abs 0.1% (=1 g/l) 1.021, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.32
This classifies the protein as stable.
Aliphatic index: 127.76
Grand average of hydropathicity (GRAVY): 0.826