ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKMQTLNFTS SLLFLSFIFL SCALLISSQQ SPSQPHSEPP SQLPFEPPVE SPFFPPSQPP 

        70         80         90        100        110        120 
IFVPPSQPPS LPPSQSQSPS LACKSTPYPK LCRTILNAVK SSPSDPYRYG KFTIKQCLKQ 

       130        140        150        160        170        180 
ASRLSKVITS YARRVESKPG SATAEEIGAV ADCGELSELS VNYLETVTTE LKTAQVMTAA 

       190        200        210        220        230        240 
LVEHVNSLLS GVVTNQQTCL DGLVEAKSGF AAAIGSPMGN LTRLYSISLG LVSHALNRNL 

       250        260        270        280        290        300 
KRFKASKGKI LGGGNSTYRE PLETLIKGLR KTCDNDKDCR KTSRNLGELG ETSGGSILVS 

       310        320        330        340        350        360 
KAVIVGPFKS DNFTTITDAI AAAPNNTRPE DGYFVIYARE GVYEEYIVVP INKKNLMLMG 

       370        380        390        400        410        420 
DGINKTIITG NHNVVDGWTT YNCSSFAVVG ERFMAVDVTF RNTAGPEKHQ AVALRNNAEG 

       430        440        450        460        470        480 
SSFYRCSFEG YQDTLYVHSL RQFYRECDIY GTVDFIFGNA AAIFQNCNIY ARKPMAKQKN 

       490        500        510        520        530        540 
AITAHGRLDP NQNTGISIIN CTIKAAPDLA AEPKSAMTFL GRPWKPYSRT VFMQSYISDI 

       550        560        570        580        590        600 
VQPVGWLEWN GTIGLDTIYY GEYSNFGPGA NTNQRVQWLG YNLLNLAEAM NFTVYNFTMG 

       610 
DTWLPQTDIP FYGGLLSKE 

References and documentation are available.

Number of amino acids: 619

Molecular weight: 67956.36

Theoretical pI: 8.52

Amino acid composition: 
Ala (A)  45	  7.3%
Arg (R)  25	  4.0%
Asn (N)  38	  6.1%
Asp (D)  21	  3.4%
Cys (C)  13	  2.1%
Gln (Q)  25	  4.0%
Glu (E)  30	  4.8%
Gly (G)  45	  7.3%
His (H)   7	  1.1%
Ile (I)  35	  5.7%
Leu (L)  53	  8.6%
Lys (K)  32	  5.2%
Met (M)  12	  1.9%
Phe (F)  29	  4.7%
Pro (P)  41	  6.6%
Ser (S)  56	  9.0%
Thr (T)  44	  7.1%
Trp (W)   6	  1.0%
Tyr (Y)  26	  4.2%
Val (V)  36	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      3037
Hydrogen    H	      4733
Nitrogen    N	       809
Oxygen      O	       911
Sulfur      S	        25

Formula: C3037H4733N809O911S25
Total number of atoms: 9515

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72490
Abs 0.1% (=1 g/l)   1.067, assuming all pairs of Cys residues form cystines


Ext. coefficient    71740
Abs 0.1% (=1 g/l)   1.056, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.93
This classifies the protein as stable.



Aliphatic index: 79.58

Grand average of hydropathicity (GRAVY): -0.209