ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKNLVVFGTE SHPKLTESIC EHLCLDIGRV ELSKFSNGET SVRIKQSVRG CDVYIVSPAS 

        70         80         90        100        110        120 
GQVNDHLMEL LIMISACKTA SAKKVTAVLP VFPYSRQPDQ KFSFSGAPLS DLQDAVVPCK 

       130        140        150        160        170        180 
KQTGYHPWIA QSGTLVADLL MCSGADHIIT MDLHDPQFQG FFDIPVDNLF GRPLLKHYIS 

       190        200        210        220        230        240 
LNIPNYHNAV IVSPDAGGAK RATAIADALG LDFALIHKNR RHEYGTSLML VGDVQNKVAI 

       250        260        270        280        290        300 
LIDDLIDTAY TLVRAAEFVK EHGASKIYAL VTHCVLSGDA IERVKLSCID KLIVTNTAPQ 

       310        320        330        340 
TITPSGCFDI IDVAPTFAEA IRRIHNGESI SILYDHNQVW V 

References and documentation are available.

Number of amino acids: 341

Molecular weight: 37338.02

Theoretical pI: 6.14

Amino acid composition: 
Ala (A)  29	  8.5%
Arg (R)  12	  3.5%
Asn (N)  12	  3.5%
Asp (D)  24	  7.0%
Cys (C)   9	  2.6%
Gln (Q)  12	  3.5%
Glu (E)  12	  3.5%
Gly (G)  20	  5.9%
His (H)  14	  4.1%
Ile (I)  29	  8.5%
Leu (L)  32	  9.4%
Lys (K)  18	  5.3%
Met (M)   6	  1.8%
Phe (F)  13	  3.8%
Pro (P)  16	  4.7%
Ser (S)  25	  7.3%
Thr (T)  18	  5.3%
Trp (W)   2	  0.6%
Tyr (Y)   9	  2.6%
Val (V)  29	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1670
Hydrogen    H	      2649
Nitrogen    N	       449
Oxygen      O	       490
Sulfur      S	        15

Formula: C1670H2649N449O490S15
Total number of atoms: 5273

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24910
Abs 0.1% (=1 g/l)   0.667, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.654, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.78
This classifies the protein as stable.



Aliphatic index: 102.93

Grand average of hydropathicity (GRAVY): 0.111