ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKPFQGITFC PTAINNEILA KKISKKIIKL GGIFSKDLTR QVNVLVVGST TNTNKFKFAV 

        70         80         90        100        110        120 
KHRFDIIFID IQAIDDIYQL WLSGENILPD SNTATMTGST YEMLKILYRR FSFKYLHNFN 

       130        140        150        160        170        180 
IFIGRITDTN ITSIDSLVRS IKKLGCSSYN YQNFVIKDTS SHNDDDDQGQ NGQISIFVTD 

       190        200        210        220        230        240 
TLLGARVNAA IEQNIPIVHF KWILDCQKRS ALLPYDPYYL LPNIKDLPYD SIGSNSCDCW 

       250        260        270        280        290        300 
DKINTTFPTN IDAQSSLQRQ QSSSTLTPSL PKTSSLLNKF KPKGEKIWDK AMSLQQHSKT 

       310        320        330        340        350        360 
NFSVLGQSPL SINNKQEDLS DNSTLIFKNC AFIIHHIFPG NHRSILTKIV VQNGGKIETS 

       370        380        390        400        410        420 
YLSGIYDHSY YIIPSNKALD SFNDLPEIID DNDGIVTEFF IERCLYYQKL LHPIDLWSKP 

       430        440        450        460        470        480 
FLSTIEFQVS SSSKLLHHEF SSSPFLNVTI TGFSGVELLH LTKVLNLLKP MGINYVEYLN 

       490        500        510        520        530        540 
KSTDILLINL AALPSIPKTH PLWSNEFSDL FTQFCINNNN DDPGDNNRKD FQNNSILRNS 

       550        560        570        580        590        600 
MKRKIEYIKK FHSIPVVTPA FIFKLLSAAS GENNEIFLNN IKWCIICPRG HKDDFKCKIK 

       610        620        630        640        650        660 
KPYYTSISSE KKYQNNDPKI DKTILLKRNN SSLSEHSMKD TKNELLQKIR ETDSGRKKRS 

       670        680        690        700        710        720 
VSSSIMDVSS ERQMPDTKRI KLESLPKNFV PKQIKRTTSW GTIMSENVPT EQPTAISNPE 

       730        740        750        760 
EIPRTEEVSH TQVTYGSIQD KKRTASLEKP MRRQTRNQTK ELDS 

References and documentation are available.

Number of amino acids: 764

Molecular weight: 87240.64

Theoretical pI: 9.08

Amino acid composition: 
Ala (A)  20	  2.6%
Arg (R)  27	  3.5%
Asn (N)  59	  7.7%
Asp (D)  47	  6.2%
Cys (C)  11	  1.4%
Gln (Q)  32	  4.2%
Glu (E)  32	  4.2%
Gly (G)  28	  3.7%
His (H)  18	  2.4%
Ile (I)  77	 10.1%
Leu (L)  68	  8.9%
Lys (K)  69	  9.0%
Met (M)  11	  1.4%
Phe (F)  38	  5.0%
Pro (P)  37	  4.8%
Ser (S)  81	 10.6%
Thr (T)  51	  6.7%
Trp (W)   8	  1.0%
Tyr (Y)  23	  3.0%
Val (V)  27	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 96

Atomic composition:

Carbon      C	      3906
Hydrogen    H	      6175
Nitrogen    N	      1049
Oxygen      O	      1169
Sulfur      S	        22

Formula: C3906H6175N1049O1169S22
Total number of atoms: 12321

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78895
Abs 0.1% (=1 g/l)   0.904, assuming all pairs of Cys residues form cystines


Ext. coefficient    78270
Abs 0.1% (=1 g/l)   0.897, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.24
This classifies the protein as unstable.



Aliphatic index: 86.88

Grand average of hydropathicity (GRAVY): -0.448