ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKPIVCDKGH GMVQAGFAGD EAPKVVFPCV VGRPRDGLNP NESYVGEEGH ANRDILTLKD 

        70         80         90        100        110        120 
PMEHGIVNNW DDMEKIWHYT FYNELRVDPE EHPVLLTEAP YNPKANREKM TQIMFESFDV 

       130        140        150        160        170        180 
PAMYVSMQSV LYLYSSGRTT GVVLDLGERV SHTVPVYEGY ALPHGILRLD LGGRDLTDYL 

       190        200        210        220        230        240 
IEIMTERGYT YTTSAEREIV RDIKEKLCYV ALDYEQEMEK TTKGWTIDKT YVLPDGQEIT 

       250        260        270        280        290        300 
IEAERFMCPE VLFQPSVIGK ESSGIHEATR NSILKCPVDT RRDMYGNILM TGGTTMLHGI 

       310        320        330        340        350        360 
KERMTKELNA LVPSSMKVKV VVPPESECSV WIGGSILASL STFHQMWITK DEYEEHGAAI 


VHRKCV 

References and documentation are available.

Number of amino acids: 366

Molecular weight: 41422.37

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  16	  4.4%
Arg (R)  18	  4.9%
Asn (N)  11	  3.0%
Asp (D)  20	  5.5%
Cys (C)   7	  1.9%
Gln (Q)   7	  1.9%
Glu (E)  35	  9.6%
Gly (G)  28	  7.7%
His (H)  12	  3.3%
Ile (I)  22	  6.0%
Leu (L)  27	  7.4%
Lys (K)  20	  5.5%
Met (M)  17	  4.6%
Phe (F)   8	  2.2%
Pro (P)  20	  5.5%
Ser (S)  19	  5.2%
Thr (T)  25	  6.8%
Trp (W)   5	  1.4%
Tyr (Y)  17	  4.6%
Val (V)  32	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1835
Hydrogen    H	      2873
Nitrogen    N	       487
Oxygen      O	       556
Sulfur      S	        24

Formula: C1835H2873N487O556S24
Total number of atoms: 5775

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53205
Abs 0.1% (=1 g/l)   1.284, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.275, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.16
This classifies the protein as stable.



Aliphatic index: 81.94

Grand average of hydropathicity (GRAVY): -0.323