ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKPPLNMSRS NKPLTQEANS SAHIDRAHQL AQDFNSKQDD TALTSLPHKN PDIFRFENNI 

        70         80         90        100        110        120 
TAHSSRRGSL YRDSDATVVL PLSEHTPRLS MDDPYRQLLQ QAEISQLRSK KKRHSSRVLR 

       130        140        150        160        170        180 
TSFISFVVLV SSLSGLDQGL ISGNVMTLSF QKYFHYPLTS PLGNIVSIVN LGAFMASLFV 

       190        200        210        220        230        240 
YSGILEPCSR KKMLQISTMI YSLGAIVQVL ALNQWCLLLG RFLLGVGMGF AFSMVIIYQF 

       250        260        270        280        290        300 
EFPLPCIRKR TLISIQCVSS VIAYSFGIWI NCAFRYLGFA WRYPLSTHVA LGIILNLMSF 

       310        320        330        340        350        360 
YLILESPSWL LKQKNDVEAL VLISNVFDDG NFEENQTQLK FRVLKRDILL KSHLQKNSYP 

       370        380        390        400        410        420 
YAYILKDFSS IIKLLIGFQL LTRTNGVDAF LYYSPLILQQ MGRGERKSIY LTGLNALIYS 

       430        440        450        460        470        480 
IVILAYVPLV LRKRKEKTNV LLGSIVMCAL LFTISFTDWF PKSTTRYISI LFAVFLFTHF 

       490        500        510        520        530        540 
ISWDSIGWVM TIELLPHLSQ APVILLVSNF YWIFKWFVSL ITPILIDRLS WKFYLIPSLS 

       550        560        570        580        590        600 
SFISIIFVLK IFPIETRDER LDSDDDSTGN GSGNHDDVFD DTGSEFSSSP SFSAYQINTL 

       610        620        630        640        650        660 
GSSIKQNNQA YSSIQNEQIL PKNGNLSNQT HGSAQNVYFI TSDSGPSRTG EFFSFHNRTD 

       670        680 
PNISDNIAAN KPSSGGGQNS PGDMAVA 

References and documentation are available.

Number of amino acids: 687

Molecular weight: 77501.39

Theoretical pI: 9.13

Amino acid composition: 
Ala (A)  32	  4.7%
Arg (R)  31	  4.5%
Asn (N)  37	  5.4%
Asp (D)  32	  4.7%
Cys (C)   6	  0.9%
Gln (Q)  31	  4.5%
Glu (E)  18	  2.6%
Gly (G)  36	  5.2%
His (H)  14	  2.0%
Ile (I)  59	  8.6%
Leu (L)  85	 12.4%
Lys (K)  30	  4.4%
Met (M)  14	  2.0%
Phe (F)  45	  6.6%
Pro (P)  30	  4.4%
Ser (S)  84	 12.2%
Thr (T)  32	  4.7%
Trp (W)  10	  1.5%
Tyr (Y)  25	  3.6%
Val (V)  36	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      3541
Hydrogen    H	      5504
Nitrogen    N	       916
Oxygen      O	       997
Sulfur      S	        20

Formula: C3541H5504N916O997S20
Total number of atoms: 10978

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    92625
Abs 0.1% (=1 g/l)   1.195, assuming all pairs of Cys residues form cystines


Ext. coefficient    92250
Abs 0.1% (=1 g/l)   1.190, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.57
This classifies the protein as unstable.



Aliphatic index: 101.60

Grand average of hydropathicity (GRAVY): 0.083