ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKQRFIRQFT NLMSTSRPKV VANKYFTSNT AKDVWSLTNE AAAKAANNSK NQGRELINLG 

        70         80         90        100        110        120 
QGFFSYSPPQ FAIKEAQKAL DIPMVNQYSP TRGRPSLINS LIKLYSPIYN TELKAENVTV 

       130        140        150        160        170        180 
TTGANEGILS CLMGLLNAGD EVIVFEPFFD QYIPNIELCG GKVVYVPINP PKELDQRNTR 

       190        200        210        220        230        240 
GEEWTIDFEQ FEKAITSKTK AVIINTPHNP IGKVFTREEL TTLGNICVKH NVVIISDEVY 

       250        260        270        280        290        300 
EHLYFTDSFT RIATLSPEIG QLTLTVGSAG KSFAATGWRI GWVLSLNAEL LSYAAKAHTR 

       310        320        330        340        350        360 
ICFASPSPLQ EACANSINDA LKIGYFEKMR QEYINKFKIF TSIFDELGLP YTAPEGTYFV 

       370        380        390        400        410        420 
LVDFSKVKIP EDYPYPEEIL NKGKDFRISH WLINELGVVA IPPTEFYIKE HEKAAENLLR 

       430        440 
FAVCKDDAYL ENAVERLKLL KDYL 

References and documentation are available.

Number of amino acids: 444

Molecular weight: 50082.38

Theoretical pI: 6.17

Amino acid composition: 
Ala (A)  34	  7.7%
Arg (R)  16	  3.6%
Asn (N)  29	  6.5%
Asp (D)  16	  3.6%
Cys (C)   6	  1.4%
Gln (Q)  13	  2.9%
Glu (E)  35	  7.9%
Gly (G)  23	  5.2%
His (H)   6	  1.4%
Ile (I)  35	  7.9%
Leu (L)  40	  9.0%
Lys (K)  32	  7.2%
Met (M)   5	  1.1%
Phe (F)  25	  5.6%
Pro (P)  24	  5.4%
Ser (S)  26	  5.9%
Thr (T)  29	  6.5%
Trp (W)   5	  1.1%
Tyr (Y)  19	  4.3%
Val (V)  26	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      2280
Hydrogen    H	      3543
Nitrogen    N	       583
Oxygen      O	       663
Sulfur      S	        11

Formula: C2280H3543N583O663S11
Total number of atoms: 7080

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56185
Abs 0.1% (=1 g/l)   1.122, assuming all pairs of Cys residues form cystines


Ext. coefficient    55810
Abs 0.1% (=1 g/l)   1.114, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.53
This classifies the protein as stable.



Aliphatic index: 90.52

Grand average of hydropathicity (GRAVY): -0.191