ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL NVVVDCGLRI 

        70         80         90        100        110        120 
YLTEFSRPIP PTPSGFVVKL RKHLKAKRLT ALKQVDQDRI LVLQFADGHF YLVLEFFSAG 

       130        140        150        160        170        180 
NVILLDENRR IMALQRVVLE HENKVGQIYE MFDESLFTTN NESADESIEK NRKAEYTSEL 

       190        200        210        220        230        240 
VNEWIKAVQA KYESDITVIK QLNIQGKEGA KKKKVKVPSI HKLLLSKVPH LSSDLLSKNL 

       250        260        270        280        290        300 
KVFNIDPSES CLNLLEETDS LAELLNSTQL EYNQLLTTTD RKGYILAKRN ENYISEKDTA 

       310        320        330        340        350        360 
DLEFIYDTFH PFKPYINGGD TDSSCIIEVE GPYNRTLDKF FSTIESSKYA LRIQNQESQA 

       370        380        390        400        410        420 
QKKIDDARAE NDRKIQALLD VQELNERKGH LIIENAPLIE EVKLAVQGLI DQQMDWNTIE 

       430        440        450        460        470        480 
KLIKSEQKKG NRIAQLLNLP LNLKQNKISV KLDLSSKELN TSSDEDNESE GNTTDSSSDS 

       490        500        510        520        530        540 
DSEDMESSKE RSTKSMKRKS NEKINVTIDL GLSAYANATE YFNIKKTSAQ KQKKVEKNVG 

       550        560        570        580        590        600 
KAMKNIEVKI DQQLKKKLKD SHSVLKKIRT PYFFEKYSWF ISSEGFLVMM GKSPAETDQI 

       610        620        630        640        650        660 
YSKYIEDDDI YMSNSFNSHV WIKNPEKTEV PPNTLMQAGI LCMSSSEAWS KKISSSPWWC 

       670        680        690        700        710        720 
FAKNVSKFDG SDNSILPEGA FRLKNENDQN HLPPAQLVMG FGFLWKVKTS GNEDNGDDDE 

       730        740        750        760        770        780 
EEEEEEEEEE EEEEEEEEEE EEEKEEEEKE EEQQQDEDDS NEVNGLEKGG DSNDSTKNNS 

       790        800        810        820        830        840 
FEHDNLEKDI EKHCTISSDT DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK 

       850        860        870        880        890        900 
RGKLKKIQKK YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVRE DRKNKREKQR 

       910        920        930        940        950        960 
RLQALKFTKK EKARVNYDKH KSELKPSLDK GDVVDDIIPV FAPWPALLKY KYKVKIQPGS 

       970        980        990       1000       1010       1020 
AKKTKTLTEI LHYFKSRPLD GSSTDNEMDW PQEHEMIKGL KEQDLVLLLC VDKLKVTIAG 

      1030 
QKSTKNGGNS SKKGKKKR 

References and documentation are available.

Number of amino acids: 1038

Molecular weight: 119069.16

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  45	  4.3%
Arg (R)  39	  3.8%
Asn (N)  67	  6.5%
Asp (D)  74	  7.1%
Cys (C)   7	  0.7%
Gln (Q)  48	  4.6%
Glu (E) 113	 10.9%
Gly (G)  44	  4.2%
His (H)  15	  1.4%
Ile (I)  63	  6.1%
Leu (L) 102	  9.8%
Lys (K) 127	 12.2%
Met (M)  16	  1.5%
Phe (F)  31	  3.0%
Pro (P)  31	  3.0%
Ser (S)  90	  8.7%
Thr (T)  43	  4.1%
Trp (W)  10	  1.0%
Tyr (Y)  26	  2.5%
Val (V)  47	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 187
Total number of positively charged residues (Arg + Lys): 166

Atomic composition:

Carbon      C	      5224
Hydrogen    H	      8401
Nitrogen    N	      1437
Oxygen      O	      1687
Sulfur      S	        23

Formula: C5224H8401N1437O1687S23
Total number of atoms: 16772

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94115
Abs 0.1% (=1 g/l)   0.790, assuming all pairs of Cys residues form cystines


Ext. coefficient    93740
Abs 0.1% (=1 g/l)   0.787, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.20
This classifies the protein as unstable.



Aliphatic index: 79.46

Grand average of hydropathicity (GRAVY): -0.871