ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRAMDGAKN EAKKSKDTDL FGRPLKKTGS WESKDNGDLM IFTHSECEGK EKIAAFDMDG 

        70         80         90        100        110        120 
TLIKTKSGKV FPTNCQDWQL LYDSIPSDFK KLHSDGFKIV IFTNQKGIHA GKVDRNEFRK 

       130        140        150        160        170        180 
KIEAIVGKLG IPVQAFVSVA GGHYRKPCVG MWNELKLRND EVEINEKESI FVGDAAGRIK 

       190        200        210        220        230        240 
TTSRPKKDHS YADRFFAANV GVKFQTPEEF FGKSKVDEPW GPPNFDPKNL FSEEITELEP 

       250        260        270        280        290        300 
HDAQLKSSEK EIILMVGFPG SGKSTFAKML GHQHDYKIVN RDTIGTWQKC VAATRSYLAD 

       310        320        330        340        350        360 
GKSVVIDNTS PDLESRKRYI DVAKELGVPI RCFEMNCSME HAQHNIRFRV LTDDNAAEIS 

       370        380        390        400 
SMVLRIHKGK YVEPTLSEGF SQIVKVNFRP KFEVEEHEKL YKMYLIE 

References and documentation are available.

Number of amino acids: 407

Molecular weight: 46187.77

Theoretical pI: 8.49

Amino acid composition: 
Ala (A)  23	  5.7%
Arg (R)  18	  4.4%
Asn (N)  17	  4.2%
Asp (D)  27	  6.6%
Cys (C)   6	  1.5%
Gln (Q)  10	  2.5%
Glu (E)  33	  8.1%
Gly (G)  29	  7.1%
His (H)  12	  2.9%
Ile (I)  26	  6.4%
Leu (L)  23	  5.7%
Lys (K)  46	 11.3%
Met (M)  11	  2.7%
Phe (F)  24	  5.9%
Pro (P)  17	  4.2%
Ser (S)  27	  6.6%
Thr (T)  18	  4.4%
Trp (W)   5	  1.2%
Tyr (Y)   9	  2.2%
Val (V)  26	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2061
Hydrogen    H	      3233
Nitrogen    N	       563
Oxygen      O	       609
Sulfur      S	        17

Formula: C2061H3233N563O609S17
Total number of atoms: 6483

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41285
Abs 0.1% (=1 g/l)   0.894, assuming all pairs of Cys residues form cystines


Ext. coefficient    40910
Abs 0.1% (=1 g/l)   0.886, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.90
This classifies the protein as stable.



Aliphatic index: 71.13

Grand average of hydropathicity (GRAVY): -0.576