ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY FMARGSVPLK 

        70         80         90        100        110        120 
SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN VIKIAQKFLY TTIGSQRLLK 

       130        140        150        160        170        180 
QDASLPELKK FLLSLLILQR GIQLDQAISD IIQRFLLTQK TMVIDLINSI FSRMVIMNMH 

       190        200        210        220        230        240 
EEAVYKWVKW MKLVNGHCEF TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA 

       250        260        270        280        290        300 
IASQFATTLL FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST 

       310        320        330        340        350        360 
YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG ILMYTMARIG 

       370        380        390        400        410        420 
ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC FSHFELFKKY DITPSTATHT 

       430        440        450        460        470        480 
IMLKVYRGLN DLDGAFRILK RLSEDPSVEI TEGHFALLIQ MCCKTTNHLI AQELFNLMTE 

       490        500        510        520        530        540 
HYNIQHTGKS ISALMDVYIE SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN 

       550        560        570        580        590        600 
ANKCEELFDK ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF 

       610        620        630        640        650        660 
EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN EEIKETINMV 

       670        680        690        700        710        720 
EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA LELYNRYNEL FFKKHDWISN 

       730        740        750        760        770        780 
NNKFVMMRSL LVLLAQIEQW KDFETLFAKY MDRIENIENL PSSTTPNIKL RSIFSGLFPY 

       790        800        810        820        830        840 
KVSQLIAMNK IDELPLLWKK LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH 

       850        860        870        880        890        900 
GYNLIHKFRL LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY 

       910        920        930        940        950        960 
LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF KELRKSKRVL 


PVSKF 

References and documentation are available.

Number of amino acids: 965

Molecular weight: 112646.06

Theoretical pI: 9.34

Amino acid composition: 
Ala (A)  51	  5.3%
Arg (R)  43	  4.5%
Asn (N)  55	  5.7%
Asp (D)  48	  5.0%
Cys (C)  11	  1.1%
Gln (Q)  40	  4.1%
Glu (E)  48	  5.0%
Gly (G)  30	  3.1%
His (H)  26	  2.7%
Ile (I)  74	  7.7%
Leu (L) 115	 11.9%
Lys (K)  86	  8.9%
Met (M)  36	  3.7%
Phe (F)  43	  4.5%
Pro (P)  28	  2.9%
Ser (S)  71	  7.4%
Thr (T)  54	  5.6%
Trp (W)  14	  1.5%
Tyr (Y)  50	  5.2%
Val (V)  42	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 129

Atomic composition:

Carbon      C	      5112
Hydrogen    H	      8045
Nitrogen    N	      1341
Oxygen      O	      1428
Sulfur      S	        47

Formula: C5112H8045N1341O1428S47
Total number of atoms: 15973

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   152125
Abs 0.1% (=1 g/l)   1.350, assuming all pairs of Cys residues form cystines


Ext. coefficient   151500
Abs 0.1% (=1 g/l)   1.345, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.54
This classifies the protein as stable.



Aliphatic index: 94.29

Grand average of hydropathicity (GRAVY): -0.264