ProtParam
User-provided sequence:
10 20 30 40 50 60
MKRFNVSYVE VIKNGETISS CFQPFQKNEN YGTITSANEQ ITPVIFHNLI MDMVLPKVVP
70 80 90 100 110 120
IKGNKVTKMS MNLIDGFDCF YSTDDHDPKT VYVCFTLVDM PKILPIRILS GLQEYESNAT
130 140 150 160 170 180
NELLSSHVGQ ILDSFHEELV EYRNQTLNSS GNGQSSNGNG QNTISDIGDA TEDQIKDVIQ
190 200 210 220 230 240
IMNDNIDKFL ERQERVSLLV DKTSQLNSSS NKFRRKAVNI KEIMWWQKVK NITLLTFTII
250
LFVSAAFMFF YLW
References and
documentation are available.
Number of amino acids: 253
Molecular weight: 28964.09
Theoretical pI: 5.44
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1294
Hydrogen H 2029
Nitrogen N 337
Oxygen O 392
Sulfur S 12
Formula: C
1294H
2029N
337O
392S
12
Total number of atoms: 4064
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27055
Abs 0.1% (=1 g/l) 0.934, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.930, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.16
This classifies the protein as unstable.
Aliphatic index: 90.43
Grand average of hydropathicity (GRAVY): -0.214