ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRFNVSYVE VIKNGETISS CFQPFQKNEN YGTITSANEQ ITPVIFHNLI MDMVLPKVVP 

        70         80         90        100        110        120 
IKGNKVTKMS MNLIDGFDCF YSTDDHDPKT VYVCFTLVDM PKILPIRILS GLQEYESNAT 

       130        140        150        160        170        180 
NELLSSHVGQ ILDSFHEELV EYRNQTLNSS GNGQSSNGNG QNTISDIGDA TEDQIKDVIQ 

       190        200        210        220        230        240 
IMNDNIDKFL ERQERVSLLV DKTSQLNSSS NKFRRKAVNI KEIMWWQKVK NITLLTFTII 

       250 
LFVSAAFMFF YLW 

References and documentation are available.

Number of amino acids: 253

Molecular weight: 28964.09

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)   6	  2.4%
Arg (R)   7	  2.8%
Asn (N)  22	  8.7%
Asp (D)  15	  5.9%
Cys (C)   3	  1.2%
Gln (Q)  13	  5.1%
Glu (E)  14	  5.5%
Gly (G)  11	  4.3%
His (H)   4	  1.6%
Ile (I)  23	  9.1%
Leu (L)  20	  7.9%
Lys (K)  17	  6.7%
Met (M)   9	  3.6%
Phe (F)  15	  5.9%
Pro (P)   7	  2.8%
Ser (S)  22	  8.7%
Thr (T)  16	  6.3%
Trp (W)   3	  1.2%
Tyr (Y)   7	  2.8%
Val (V)  19	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1294
Hydrogen    H	      2029
Nitrogen    N	       337
Oxygen      O	       392
Sulfur      S	        12

Formula: C1294H2029N337O392S12
Total number of atoms: 4064

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27055
Abs 0.1% (=1 g/l)   0.934, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.930, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.16
This classifies the protein as unstable.



Aliphatic index: 90.43

Grand average of hydropathicity (GRAVY): -0.214